犊牦牛血清和组织中一氧化氮含量测定

测定1日龄和1月龄犊牦牛血清和肺、肾、骨骼肌、心肌、脾中NO含量,结果分别为:97.68±29.69和64.47±7.46(μmol/L),2.32±0.45和3.12±0.78(μmol/gprot,下同),3.04±0.53和5.36±1.22,3.03±1.10和4.49±0.83,5.87±2.41和6.31±2.92,1.56±0.25和1.72±0.72。结果表明,血清中NO的含量1日龄显著高于1月龄犊牦牛(P<0.05)。     

不同发育期牦牛血清钙、无机磷和碱性磷酸酶的测定及分析

对同一海拔地区不同发育期牦牛血清钙、无机磷含量和碱性磷酸酶活性进行测定。结果:1日龄和1月龄血清钙含量较高,6月龄和成年组牦牛血清钙含量降低,且明显低于普通牛(P<0.05),导致血清钙与无机磷之比较低;6月龄血清无机磷含量显著低于其余年龄组(P<0.05);游离钙由1日龄的1.48±0.16 mmol/L降低到成年组的0.43±0.02 mmol/L(P<0.05);幼龄犊牦牛碱性磷酸酶显著高于成年组,由1日龄的708.1±202.6 U/L降低到成年组的267.2±123.5 U/L(P<0.05)。结果表明:犊牦牛在生长发育期普遍存在缺钙和血清钙与无机磷比例失调的问题。     

高原鼢鼠心肌和骨骼肌肌红蛋白含量的季节变化

为了研究高原鼢鼠肌红蛋白在不同季节的变化,用分光光度法测定了肌红蛋白含量。结果显示:在春季、夏季和秋季,高原鼢鼠心肌肌红蛋白含量分别为1 001.25±103.25(nmol/g),726.50±54.70(nmol/g),767.33±88.73(nmol/g);骨骼肌肌红蛋白含量分别为781.85±100.98(nmol/g),672.15±108.88(nmol/g),676.80±77.31(nmol/g)。春季高原鼢鼠心肌肌红蛋白和骨骼肌肌红蛋白含量显著高于夏季和秋季(P<0.05)。说明随着洞道氧气和二氧化碳浓度的季节性变化,高原鼢鼠通过改变肌红蛋白含量,来维持机体的氧平衡。     

不同海拔地区牦牛心肌、骨骼肌线粒体GSH-PX的测定

对青海省玛多县、刚察县牦牛及甘肃黄牛的心肌和骨骼肌线粒体内谷胱甘肽过氧化物酶(GSH-PX)活性进行了测定.其心肌、骨骼肌的GSH-PX玛多牦牛分别为:(20.15±5.43)、(15.32±4.27)U/mg prot;刚察牦牛分别为:(6.67±5.67)、(7.76±4.83)U/mg prot;甘肃黄牛分别为:...     

牦牛肌红蛋白的cDNA序列测定及其氧化特性的研究

通过对牦牛肌红蛋白的含量和cDNA序列的测定及心肌肌红蛋白氧化和脂类氧化相关性的研究，初步探讨了牦牛适应高原低氧环境的机理. 牦牛与黄牛的肌红蛋白cDNA序列比较，二者只有2个碱基的差异（89位氨基酸由cac变为cat，91位氨基酸由gcc变为gct），但由于氨基酸密码子简并性，两者的氨基酸序列没有差异.牦牛的骨骼肌的肌红蛋白含量为488.3 nmol/g，与黄牛相比没有显著差异，但牦牛心肌的肌红蛋白含量为823.4 nmol/g，比黄牛高61.2％（P＜0.05）.在4 ℃贮存下，牦牛心肌中肌红蛋白的氧     

超声辅助提取冷冻牦牛血中血红素的工艺优化

为了提高冷冻牦牛血液中血红素的得率,增加牦牛血液资源的利用价值。文中采用超声辅助提取法(ultrasound assisted extraction,UAE)研究了超声温度、超声功率和超声时间3个因素对血红素得率的影响,通过响应面分析优化出超声辅助提取冷冻牦牛血液中血红素的最佳工艺。最优工艺条件为超声时间10. 27 min,超声温度29. 41℃,超声比功率383. 28 W/100 m L,在此条件下血红素的得率为(0. 296±0. 021) g/100 m L,与预测值(0. 280±0. 017) g/100 m L相差不显著,验证了以牦牛血红素得率Y为响应值的多元二次回归模型的可靠性。采用超声辅助提取法能有效增加冷冻牦牛血液中血红素的提取效率。     

青海玛多和循化牦牛血浆及组织中SOD活性的研究

对青海省玛多县和循化县两地牦牛的血浆和5个组织中的SOD的活性进行了测定,结果:玛多牦牛的各项指标分别为血浆(199 72±9 81)NU/mL,心肌(46 00±14 79)NU/mgprot,骨骼肌(31 96±13 40)NU/mgprot,肺(33 37±5 57)NU/mgprot,肾(24 19±4 77)NU/mgprot,肝脏(15 84±2 47)NU/mgprot;循化牦牛分别为血浆(181 32±21 22)NU/mL,心肌(47 98±4 68)NU/mgprot,肾(43.00±11 02)NU/mgprot,骨骼肌(35 54±7 89)NU/mgprot,肺(26 83±4 46)NU/mgprot,肝脏(10 71±2 78)NU/mgprot。玛多牦牛的血浆、肝、肺组织的SOD活性极显著的高于循化县牦牛(P<0 01或P<0 05)。     

牦牛血清和组织中一氧化氮合成酶活性的测定

对生活在海拔4300多m玛多牦牛和3500m左右循化牦牛血清和肝、肺、肾、心肌、骨骼肌中的一氧化氮合成酶(NOS)活性进行了测定,结果分别为:(28 21±2 99)U/mL和(24 35±4 64)U/mL,(2 05±0 70)U/mgprot和(2 55±0 58)U/mgprot,(0 98±0 56)U/mgprot和(1 52±0 40)U/mgprot,(0 72±0 35)U/mgprot和(1 61±0 57)U/mgprot,(0 73±0 46)U/mgprot和(0 70±0 21)U/mgprot,(1 10±0 73)U/mgprot和(0 66±0 33)U/mgprot。     

血液检验红细胞参数在贫血鉴别诊断中的应用效果观察

分析贫血鉴别诊断中血液检验红细胞参数的具体价值。选取2020年4月—2021年5月兰州市第二人民医院血液内科接收的贫血患者62例,将其设定为观察组,同期随机抽取62例健康体检者,将其设定为对照组,检验所有受检者的血液红细胞参数,并进一步鉴别患者的不同贫血类型。62例贫血患者中,缺铁性贫血38例,溶血性贫血24例,其中缺铁性贫血患者的血红蛋白(Hb)(75.69±0.71)g/L、红细胞数量(RBC)(3.85±0.92)×10¹²/L,溶血性贫血患者的Hb、RBC分别为(1.03±0.62)g/L、(2.09±0.87)×10¹²/L,组别差异较大,经检验有统计学意义(P<0.05)。缺铁性贫血患者的平均红细胞体积(MCV)(52.64±1.31)fl、平均红细胞血红蛋白含量(MCH)(20.15±1.52)pg/L、红细胞分布密度(RDW)(17.86±1.13)%,溶血性贫血患者的MCV、MCH、RDW分别为(77.19±1.25)fl、(25.47±1.24)pg/L、(22.96±1.08)%,组别之间的差异比较大,检验有统计学...     

不同海拔地区牦牛心肌、骨骼肌线粒体SOD活性的测定

对青海省玛多、刚察两县牦牛两种组织线粒体中SOD活性进行测定,结果：玛多、刚察牦牛心肌、骨骼肌线粒体中SOD活性分别为58．42±8．10U／mg,61．86±6．61U／mg;42．95±4．87U／mg,44．56±20．46U／mg。玛多牦牛心肌,骨骼肌组织线粒体中SOD活性显著高于刚察牦牛（P〈0.05）。结论：在高原低氧环境下,牦牛组织线粒体中SOD的活性随海拔高度的升高而增强。     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.