不同生境下两种表型盐地碱蓬叶片POD比较研究

以不同自然生境下两种表型盐地碱蓬叶片为材料,分析了H2O2含量、MDA含量、质膜透性、过氧化物酶（POD）的活性及其同工酶．结果表明：紫红色表型盐地碱蓬叶片H2O2含量是绿色表型的2．48倍,而其MDA含量和质膜相对透性却显著低于后者．绿色表型盐地碱蓬POD活性显著高于紫红色表型盐地碱蓬,同工酶分析表明,紫红色表型盐地碱蓬叶片POD同工酶有6条带,即POD1、POD2、POD3、POD4、POD5、POD6,绿色表型盐地碱蓬叶片有5条同工酶条带,为POD1、POD2、POD3、POD4、POD5,POD1是两种表型盐地碱蓬叶片的主要同工酶,占POD总活性的60％以上．绿色表型盐地碱蓬叶片POD1、POD2和POD3的活性分别为紫红色表型的1．39倍、4．11倍和3．21倍．这些结果表明,与绿色表型盐地碱蓬相比,紫红色表型盐地碱蓬叶片POD活性低,可能是其叶片积累更高H2O2原因．     

紫红色表型盐地碱蓬叶片营养成分分析

本文分析了黄河三角州海边潮间带叶片的营养成分.结果表明,与绿色表型叶片相比,紫红色表型盐地碱蓬叶片中粗蛋白、粗纤维、粗脂肪、总糖含量较低,而甜菜红素、维生素c和类胡萝卜素等抗氧化剂成分含量较高.这些结果为紫红色表型盐地碱蓬利用提供了依据     

不同自然盐渍生境下盐地碱蓬叶片色素积累及光合特性的研究

本文以黄河三角洲内地重盐碱地和海边潮间带两种乍境下的盐地碱蓬为材料,比较不同生境对盐地碱蓬色素积累和相关光合指标的影响.结果表明:海边生境的盐地碱蓬叶片中甜菜红素的含量显著高于内地生境条件下的,而叶绿素a、叶绿素b的含量显著低于内地生境条件下的.内地生境盐地碱蓬功能叶片净光合速率,Fu/Fm,ΦPSⅡ均显著高于海边生境的功能叶片.说明海边生境的盐地碱蓬可能通过调节叶片中不同色素的含量,改变光合特性,适应综合逆境.     

不同自然盐渍生境下盐地碱蓬叶片肉质化研究

以黄河三角洲内地重盐碱地和海边潮问带两种生境下的盐地碱蓬为材料，比较叶片肉质化及叶片细胞液和土壤中离子浓度等内外因索，探讨影响盐地碱蓬叶片肉质化的主要因素．结果表明：内地生境条件下绿色盐地碱蓬叶片肉质化程度显著高于海边生境条件下的红色盐地碱蓬．叶片肉质化程度与土壤含水量、土壤中Na^＋、Cl^＋含量和叶片中Na^＋、Cl^-含量呈显著正相关。相关系敷：叶片中Na^＋、Cl^-含量〉土壤中Na^＋、Cl^-含量〉土壤含水量；Na^＋〉Cl^-．表明白然生境下叶片中Na^＋、Cl^-积累是盐地碱蓬肉质化主要因素，而Na^＋起主导作用．内地重盐碱地土壤表层和空气温度高，尤其土壤含水量低是内地盐地碱蓬叶片肉质化程度高于海边生境条件下的重要原因．     

盐地碱蓬超氧化物歧化酶的提取及抗氧化活性的研究

以盐地碱蓬为原料提取超氧化物歧化酶(SOD),采用邻苯三酚自氧化法对其酶活进行测定。实验结果表明:10g盐地碱蓬中提取的SOD,经硫酸铵分级沉淀、氯仿/乙醇沉淀、丙酮沉淀及热处理后,酶活分别为427.035U/mg、648.6736U/mg和1181.856U/mg。     

NaCl胁迫下两种表型盐地碱蓬幼苗叶片中色素含量的比较

为研究自然条件下海边生境盐地碱蓬和内地生境盐地碱蓬对环境的适应性能否遗传,我们以两种表型盐地碱蓬为实验材料,测定了不同浓度NaCl处理下其叶片中甜菜红素、叶绿素a、叶绿素b和类胡萝卜素的含量,并计算总叶绿素(chla+chlb)含量及Chla/b.结果表明:不同浓度NaCl处理下,海边生境盐地碱蓬叶片中甜菜红素含量及Chla/b比值始终高于内地生境的,而内地生境盐地碱蓬叶片中叶绿素a、叶绿索b、总叶绿素和类胡萝卜素的含量均高于海边生境的.这表明两种表型盐地碱蓬对自然环境长期适应的结果,具有一定可遗传性.     

盐地碱蓬甲硫氨酸合成酶基因(SsMS)的克隆与表达分析

从盐地碱蓬 (Suaedasalsa)中克隆了甲硫氨酸合成酶基因的cDNA片段 .通过Southern杂交和Northern杂交对其表达特性进行分析 ,结果表明 ,同一盐浓度不同时间处理下、双氧水及冷害刺激条件下甲硫氨酸合成酶在碱蓬叶中的表达量先呈现减少然后增加的趋势 ;聚乙二醇 (PEG)透导下呈现下降趋势 .由此可见该酶在盐、氧化和低温胁迫条件下产生一定的反应 .     

外源苯丙烯酸对黄瓜叶片弱光胁迫下抗坏血酸-谷胱甘肽循环系统的影响

以"津春4号"黄瓜品种为材料,研究外源苯丙烯酸对弱光下黄瓜幼苗叶片抗坏血酸-谷胱甘肽(ASA-GSH)循环中抗氧化酶活性和非酶抗氧化物含量以及活性氧代谢的影响.研究结果表明,用50μmol苯丙烯酸(CA)处理2天后,MDA和H_2O_2含量降低,脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏血酸还原酶(MDHAR)、抗坏血酸过氧化物酶(APX)和谷胱甘肽还原酶(GR)活性提高,还原型抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量增加.8天后,弱光胁迫显著提高了MDA和H_2O_2含量,提高了DHAR,MDHAR,APX和GR的活性以及还原型抗坏血酸(AsA)和还原型谷胱甘肽(GSH)含量;CA预处理则降低了弱光胁迫下MDA和H_2O_2含量,提高了DHAR,MDHAR,APX和GR的活性以及AsA和GSH含量.外源CA预处理通过抗坏血酸-谷胱甘肽循环缓解了弱光下黄瓜叶片的膜脂过氧化损伤.     

盐地碱蓬甲硫氨酸合成酶基因（MsMS）的克隆与表达分析

从盐地碱蓬(Suaeda salsa)中克隆了甲硫氨酸合成酶基因的cDNA片段．通过Southem杂交和Notthem杂交对其表达特性进行分析，结果表明，同一盐浓度不同时间处理下、双氧水及冷害刺激条件下甲硫氨酸合成酶在碱蓬叶中的表达量先呈现减少然后增加的趋势；聚乙二醇(PEG)透导下呈现下降趋势．由此可见该酶在盐、氧化和低温胁迫条件下产生一定的反应．     

NaCl对海滨锦葵活性氧清除能力的影响

采用沙配法,以海滨锦葵为实验材料,研究0、100、200、300mmol/LNaCl处理下,海滨锦葵活性氧清除酶系统中超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）、过氧化氢酶（CAT）等酶活性的变化,以及谷胱甘肽（GSH）、抗坏血酸（AsA）等活性氧清除物质含量的变化.结果表明,NaCl处理15天后,SOD、APX活性较对照明显升高;AsA、GSH含量均显著上升,且升幅相对较大.CAT的活性略有增加,但未见明显上升.这表明：较强的活性氧清除能力是盐胁迫下海滨锦葵的一个重要保护机制.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Features of Women's language

Language is a means of verbal communication. People use language to communicate with each other. In the society, no two speakers are exactly alike in the way of speaking. Some differences are due to age, gender, statue and personality. Above all, gender is one of the obvious reasons. The writer of this paper tries to describe the features of women＇s language from these perspectives： pronunciation, intonation, diction, subjects, grammar and discourse. From the discussion of the features of women＇s language, more attention should be paid to language use in social context. What＇s more, the linguistic phenomena in a speaking community can be understood more thoroughly.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

海南岛地体及其毗邻陆缘晚中生代—新生代古地磁研究和构造演化

本文叙述了对海南岛及其毗邻大陆边缘白垩纪到第四纪地层岩石进行古地磁研究的全部工作过程。通过分析岩石中剩余磁矢量的磁偏角及磁倾角的变化,提出海南岛白垩纪以来经历的构造演化模式如下:早期伴随顺时针旋转而向南迁移,后期伴随逆时针转动并向北运移。联系该地区及邻区的地质、地球物理资料,对海南岛上述的构造地体运动提出以下认识:北部湾内早期有一拉张作用,主要是该作用使湾内地壳显著伸长减薄,形成北部湾盆地。从而导致了海南岛的早期构造运动,而海南岛后期的构造运动则主要是受南海海底扩张的影响。海南地体运动规律的阐明对于了解北部湾油气盆地的形成演化有重要的理论和实际意义。     

An Index mechanism for multi-scale view in spatial databases

There are numerous geometric objects stored in the spatial databases. An importance function in a spatial database is that users can browse the geometric objects as a map efficiently. Thus the spatial database should display the geometric objects users concern about swiftly onto the display window. This process includes two operations:retrieve data from database and then draw them onto screen. Accordingly, to improve the efficiency, we should try to reduce time of both retrieving object and displaying them. The former can be achieved with the aid of spatial index such as R-tree, the latter require to simplify the objects. Simplification means that objects are shown with sufficient but not with unnecessary detail which depend on the scale of browse. So the major problem is how to retrieve data at different detail level efficiently. This paper introduces the implementation of a multi-scale index in the spatial database SISP (Spatial Information Shared Platform) which is generalized from R-tree. The difference between the generalization and the R-tree lies on two facets: One is that every node and geometric object in the generalization is assigned with a importance value which denote the importance of them, and every vertex in the objects are assigned with a importance value,too. The importance value can be use to decide which data should be retrieve from disk in a query. The other difference is that geometric objects in the generalization are divided into one or more sub-blocks, and vertexes are total ordered by their importance value. With the help of the generalized R-tree, one can easily retrieve data at different detail levels.Some experiments are performed on real-life data to evaluate the performance of solutions that separately use normal spatial index and multi-scale spatial index. The results show that the solution using multi-scale index in SISP is satisfying.     

《Chinese Science Bulletin》SUBJECT INDEX TO VOLUME 59(2014)

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Achieving Unusual States of Matter Under High Pressure

The elongation method,originally proposed by Imamura was further developed for many years in our group.As a method towards O(N)with high efficiency and high accuracy for any dimensional systems.This treatment designed for one-dimensional(ID)polymers is now available for three-dimensional(3D)systems,but geometry optimization is now possible only for 1D-systems.As an approach toward post-Hartree-Fock,it was also extended to