细胞分裂素对芦荟不定芽增殖的影响

在加有不同种类激素的 MS培养基上离体培养元江芦荟继代芽 ,研究激素对元江芦荟不定芽增殖的影响。结果表明 ,不同质量浓度和种类的激素对元江芦荟不定芽增殖及芽的分化和生长质量影响很大 ,最理想的培养基为 :MS+ 3%蔗糖 + 6- BA2 mg· L- 1+ KT2 mg· L- 1,增殖倍数达 1 2 .87。     

辣椒离体高效再生体系及其卡那霉素筛选体系的建立

选用辣椒(CapsicumannuumL.)4个品种的子叶以及下胚轴为外植体进行不定芽的诱导分化、生长及生根成苗试验.在组织培养的各个阶段的培养基中,附加不同种类及不同质量浓度的激素组合,观察测量外植体在各种培养基中的生长情况,从中筛选出最优的激素配比培养基,从而建立了辣椒的高效离体植株再生体系.在此基础上,将不同质量浓度梯度的卡那霉素(Kan)分别加入子叶、下胚轴愈伤组织诱导及不定芽分化阶段的培养基中,观测Kan对外植体生长的影响,以确定各阶段的最低致死质量浓度,用于辣椒的遗传转化研究.试验结果表明最佳的辣椒离体再生培养基为:芽分化阶段,MS+6-BA3.0mg·L-1+IAA0.1mg·L-1;不定芽伸长阶段,MS+GA32mg·L-1+6-BA0.5mg·L-1;诱导生根,MS+IAA1.0mg·L-1.合适的Kan质量浓度为:下胚轴出愈为80mg·L-1;子叶分化出不定芽为60mg·L-1.     

甘蔗组织培养中培养基的优化

比较了不同基本培养基 ,不同激素及其不同质量浓度的组合对甘蔗愈伤组织的诱导 ,不定芽的分化及其生根的效果 .结果表明 :MS + 2 ,4 -D 1～ 5mg·L-1培养基均可诱导愈伤组织的增殖 ;MS +KT 1.5～ 2 .0mg·L-1+ 6 -BA 2mg·L-1培养基对愈伤组织分化不定芽的效果最佳 ;1 2MS +NAA 1～ 4mg·L-1培养基对生根较好 .     

丽格海棠的叶片培养与快速繁殖

以MS为基本培养基,通过不同的激素浓度配比试验对丽格海棠丛生芽的形成、继代培养及根的诱导条件进行了初步探讨,发现以丽格海棠的叶片为外植体,采用MS培养基+NAA0.5 mg·L-1+6 BA1.0 mg·L-1,30 mg·L-1蔗糖、8 mg·L-1琼脂、pH5.8的培养基,在温度为25℃左右,光照20001 x、12h的培养条件下可以较快较多的促进丽格海棠叶片产生不定芽,芽丛不断分化、增殖,可经多次继代转接产生幼芽.幼芽生长后,可转接到生根培养基1/2MS+NAA1.0 mg·L-1诱导生根产生试管苗.     

鸡蛋枣的组织培养与快速繁殖技术

以鸡蛋枣的茎段为外植体,进行了芽的诱导和成苗的研究.结果表明:在MS+ZT1.75mg·L-1+KT2.0mg·L-1培养基,茎段可以分化不定芽.低浓度的NAA促进不定芽的形成,在MS+ZT1.75mg·L-1+KT2.0mg·L-1+NAA0.05mg·L-1中培养基芽的增殖系数达到4.2倍.在1 2MS+IBA0.8mg·L-1培养基中,试管苗的生根率达到95.0%.试验还就外植体的消毒和组培苗的移植进行了探索.     

利用从芽途径快速繁殖蝴蝶兰的研究

报道了利用从生芽途径快速繁殖蝴蝶兰的研究结果,试验表明:培养基MS+BA1.0mg·L-1+NAA0.05mg·L-1+GA30.5mg·L-1能够诱导花梗休眠芽转化为营养芽.花梗芽在培养基MS+BA5.0mg·L-1+NAA0.05mg·L-1上能够诱导产生丛生芽.在丛生芽增殖阶段,以BA12.5mg·L-1+NAA0.05mg·L-1的增殖效果最好.BA、KT、ZT均能诱导产生"丛生芽",其中以ZT和BA的诱导效果最好.另外,在增殖阶段,外植体采用双芽接较单芽接的增殖效果好.在生根阶段以1 2MS+NAA0.5mg·L-1+w=0.2%的活性炭+φ=10%的椰子水的生根效果较佳,生根率可达100%,且植株生长健壮.试管苗移栽在水苔上的成活率较高,成活率达96 7%.     

番茄高效再生体系的建立

比较了不同基本培养基,不同激素及其不同浓度组合对番茄愈伤组织的增殖、不定芽分化及其生根的效果.结果表明:MS+NAA0.02～0.10mg·L-1+6-BA3.0mg·L-1+蔗糖30g·L-1(pH=5.8)均可诱导愈伤组织的增殖,但以MS+6-BA3.0mg·L-1+NAA0.05mg·L-1+蔗糖30g·L-1最佳;MS+IAA0.1mg·L-1+6-BA2.0mg·L-1+蔗糖30g·L-1培养基对愈伤组织分化不定芽的效果最佳;MS+IAA0.1mg·L-1+蔗糖20g·L-1培养基对生根较好.     

观赏凤梨组织培养不同外植体的比较试验

选用观赏凤梨不同品种的老叶鞘、嫩叶鞘、顶芽、侧芽、茎段、叶片等不同组织作外植体 ,接种在诱导培养基为改良 MS+ 2 .0 mg· L- 1的 BA+ 5 .0 mg· L- 1的 2 ,4- D+ 0 .2 mg· L- 1的 IBA,及分化培养基为改良 MS+ 4 .0 mg· L- 1的 BA+ 0 .2 mg·L- 1的 IBA,培养 60 d进行统计 ,老叶鞘的愈伤组织三个品种平均诱导率为 74.6% ,丛生芽的诱导率平均数达 1 7.1 % ,次之是侧芽、顶芽 ,嫩叶鞘、茎段、叶片 ,后者能诱导出愈伤组织 ,不能诱导出丛生芽     

火炬姜叶片培养及植株再生

报道了火炬姜叶片离体培养的研究结果 ,试验表明 :诱导叶片产生愈伤组织效果较好的激素配比是 6 -BA 0 .5mg·L- 1+ 2 ,4 -D 0 .5mg·L- 1;最适于愈伤组织分化的基本培养基是MS ;6 -BA、ZT和KT均能诱导愈伤组织分化不定芽 ,其中以 6 -BA的效果较好和经济 ;在所试的 6 -BA质量浓度中 ,以 3mg·L- 1的效果较佳 ;添加 80 0mg·L- 1的CH有利于火炬姜愈伤组织的分化 ;生根培养阶段 ,以附加w =0 .15 %的活性炭的 1/ 2MS培养基效果较好 ,生根率达 10 0 % ,且植株生长健壮 ;试管苗移栽在表土上 ,成活率可达 10 0 %     

怀地黄不定芽诱导生根和微型块茎繁殖的研究

研究了以怀地黄(Rehmannia glutinosa)不定芽诱导生根的适宜培养基以及微型块茎繁殖.结果表明:以IBA2.0mg·L-1+BA0.5 mg·L-1,糖浓度40g·L-1培养基生根效果好,根多、粗壮,移栽后成活率可达98.2％;以IBA2.0mg·L-1+BA2.0mg·L-1+NAA0.2mg·L-1,糖浓度为40g·L-1为诱导微型块茎的最佳培养基.     

Markedness and its Interpretation in Language Use

Language markedness is a common phenomenon in languages, and is reflected from hearing, vision and sense, i.e. the variation in the three aspects such as phonology, morphology and semantics. This paper focuses on the interpretation of markedness in language use following the three perspectives, i.e. pragmatic interpretation, psychological interpretation and cognitive interpretation, with an aim to define the function of markedness.     

Lower Paleozoic oil relationships within Williston Basin, Canada

The Williston Basin is a significant petroleum province, containing oil production zones that include the Middle Cambrian to Lower Ordovician, Upper Ordovician, Middle Devonian, Upper Devonian and Mississippian and within the Jurassic and Cretaceous. The oils of the Williston Basin exhibit a wide range of geochemical characteristics defined as "oil families", although the geochemical signature of the Cambrian Deadwood Formation and Lower Ordovician Winnipeg reservoired oils does not match any "oil family". Despite their close stratigraphic proximity, it is evident that the oils of the Lower Palaeozoic within the Williston Basin are distinct. This suggests the presence of a new "oil family" within the Williston Basin. Diagnostic geochemical signatures occur in the gasoline range chromatograms, within saturate fraction gas chromatograms and biomarker fingerprints. However, some of the established criteria and cross-plots that are currently used to segregate oils into distinct genetic families within the basin do not always meet with success, particularly when applied to the Lower Palaeozoic oils of the Deadwood and Winnipeg Formation.     

An Analysis of Catherine＇s Character——Wuthering Heights

Wuthering Heights, Emily Bronte＇s only novel, was published in December of 1847 under the pseudonym Ellis Bell. The book did not gain immediate success, but it is now thought one of the finest novels in the English language. Catherine is the key character of this masterpiece, because everybody and everything center on her though she had a short life. We can understand this masterpiece better if we know Catherine well.     

Characterization of Ordovician carbonate reservoirs, southeastern Saskatchewan, Canada

The discovery of the prolific Ordovician Red River reservoirs in 1995 in southeastern Saskatchewan was the catalyst for extensive exploration activity which resulted in the discovery of more than 15 new Red River pools. The best yields of Red River production to date have been from dolomite reservoirs. Understanding the processes of dolomitization is, therefore, crucial for the prediction of the connectivity, spatial distribution and heterogeneity of dolomite reservoirs.The Red River reservoirs in the Midale area consist of 3～4 thin dolomitized zones, with a total thickness of about 20 m, which occur at the top of the Yeoman Formation. Two types of replacement dolomite were recognized in the Red River reservoir: dolomitized burrow infills and dolomitized host matrix. The spatial distribution of dolomite suggests that burrowing organisms played an important role in facilitating the fluid flow in the backfilled sediments. This resulted in penecontemporaneous dolomitization of burrow infills by normal seawater. The dolomite in the host matrix is interpreted as having occurred at shallow burial by evaporitic seawater during precipitation of Lake Almar anhydrite that immediately overlies the Yeoman Formation. However, the low δ18O values of dolomited burrow infills (-5.9‰～ -7.8‰, PDB) and matrix dolomites (-6.6‰～ -8.1‰, avg. -7.4‰ PDB) compared to the estimated values for the late Ordovician marine dolomite could be attributed to modification and alteration of dolomite at higher temperatures during deeper burial, which could also be responsible for its 87Sr/86Sr ratios (0.7084～0.7088) that are higher than suggested for the late Ordovician seawaters (0.7078～0.7080). The trace amounts of saddle dolomite cement in the Red River carbonates are probably related to "cannibalization" of earlier replacement dolomite during the chemical compaction.     

Design and analysis of generic LBS application developing platform

Location based services is promising due to its novel working style and contents.A software platform is proposed to provide application programs of typical location based services and support new applications developing efficiently. The analysis shows that this scheme is easy implemented, low cost and adapt to all kinds of mobile nework system.     

橡胶颗粒沥青混合料低温抗裂性试验研究

以AC-13级配为基础,将橡胶颗粒代替部分集料掺入混合料中,以低温弯曲试验为评价方法对不同橡胶颗粒掺量下沥青混合料的低温抗裂性进行研究,并引入应变能密度值对混合料的低温抗裂性进行综合评价.试验结果表明:橡胶颗粒沥青混合料试件的破坏微应变均超过2 300,满足冬寒区的技术指标;无论是否掺加橡胶颗粒,随着温度的下降,沥青混合料破坏时的最大弯拉强度增大,弯拉应变降低,劲度模量增大;弯曲应变能密度在胶粒掺量为1%左右时具有较大的弯曲应变能密度值,此时橡胶颗粒沥青混合料具有较好的低温抗裂性.     

低渗透非均质砂岩油藏启动压力梯度研究

理论推导与室内实验相结合,建立了低渗透非均质砂岩油藏启动压力梯度确定方法。首先借助油藏流场与电场相似的原理,推导了非均质砂岩油藏启动压力梯度计算公式。其次基于稳定流实验方法,建立了非均质砂岩油藏启动压力梯度测试方法。结果表明:低渗透非均质砂岩油藏的启动压力梯度确定遵循两个等效原则。平面非均质油藏的启动压力梯度等于各级渗透率段的启动压力梯度关于长度的加权平均;纵向非均质油藏的启动压力梯度等于各渗透率层的启动压力梯度关于渗透率与渗流面积乘积的加权平均。研究成果可用于有效指导低渗透非均质砂岩油藏的合理井距确定,促进该类油藏的高效开发。     

Quantitative trait loci （QTLs） for quality traits related to protein and starch in wheat

Quality traits in wheat （Triticum aestirum L.） were studied by quantitative trait locus （QTL） analysis in a recombinant inbred line （RIL） population, a set of 131 lines derived from Chuan 35050 × Shannong 483 cross （ChSh）. Grains from RILs were assayed for 21 quality traits related to protein and starch. A total of 35 putative QTLs for 19 traits with a single QTL explaining 7.99-40.52% of phenotypic variations were detected on 10 chromosomes, 1D, 2A, 2D, 3B, 3D, 5A, 6A, 6B, 6D, and 7B. The additive effects of 30 QTLs were positive, contributed by Chuan 35050, the remaining 5 QTLs were negative with the additive effect contributed by Shannong 483. For protein traits, 15 QTLs were obtained and most of them were located on chromosomes 1 D, 3B and 6D, while 20 QTLs for starch traits were detected and most of them were located on chromosomes 3D, 6B and 7B. Only 7 QTLs for protein and starch traits were co-located in three regions on chromosomes 1D, 2A and 2D. These protein and starch trait QTLs showed a distinct distribution pattern in certain regions and chromosomes. Twenty-two QTLs were clustered in 6 regions of 5 chromosomes. Two QTL clusters for protein traits were located on chromosomes 1D and 3B, respectively, three clusters for starch traits on chromosomes 3D, 6B and 7B, and one cluster including protein and starch traits on chromosome 1D.     

On Tragic Consciousness In The Works By Ernest Hemingway

As an American modern novelist who were famous in the literary world, Hemingway was not a person who always followed the trend but a sharp observer. At the same time, he was a tragedy maestro, he paid great attention on existence, fate and end-result. The dramatis personae's tragedy of his works was an extreme limit by all means tragedy on the meaning of fearless challenge that failed. The beauty of tragedy was not produced on the destruction of life, but now this kind of value was in the impact activity. They performed for the reader about the tragedy on challenging for the limit and the death.     

A computer generator for randomly layered structures

AcomputergeneratorforrandomlylayeredstructuresYUJia shun1,2,HEZhen hua2(1.TheInstituteofGeologicalandNuclearSciences,NewZealand;2.StateKeyLaboratoryofOilandGasReservoirGeologyandExploitation,ChengduUniversityofTechnology,China)Abstract:Analgorithmisintrod…