4,4—二氯—8,11—二甲氧基三环[5,4,0,0^3,5]十一碳—7,9…

本文报导４，４－二氯－８，１１－二甲氧基三环［４，４，０，０＾３，５］十一碳－７，９，１１－三稀的合成，用元素分析确定其分子组成为Ｃ１３Ｈ１４Ｏ２Ｃｌ２，测定并讨论其质谱，＾１Ｈ和＾１３Ｃ核磁共振谱。     

Erd■s一个猜想的注记

当ｋ≥２，２ｋｎ＋１＝ｑｈ，ｑ≡－１（ｍｏｄ２ｋ），丢番图方程４／ｎ＝ｘ－１十ｙ－１＋ｚ－１有正整数解；当方程中ｎ换以素数Ｐ，则Ｐ存疑的条件是Ｌｅｇｅｎｄｒｅ符号有（Ｐ／３）＝（Ｐ／５）＝（Ｐ／７）＝（Ｐ／１１）＝（Ｐ／１３）＝（Ｐ／１７）＝１．     

两种二茂铁甲基二氧大环多胺的电化学性质

利用循环伏安法对我们新设计合成的两种二茂铁甲基二氧大环多胺化合物－１－二茂铁甲基－１，４，８，１１－四氮杂环十三烷－５，７－二酮和１－二茂铁甲基－１，４，７，１１，１４－五氮杂环十六烷－３，１５－二酮－的电化学性质进行了研究，包括酸碱度的影响以及它们的Ｚｎ＾２＋，Ｃａ＾２＋，Ｃｄ＾２＋，Ｃｕ＾２＋Ｎｉ＾２＋，Ｃｏ＾２＋等金属离子的电化学响应情况，在乙醇和水的混合溶剂体中化合物ＦｃＣＨ２Ｌ１对Ｎｉ＾     

小鼠肺腺癌细胞系（LA－795）高、低转移亚系染色体核型比较研究

对小鼠肺腺癌细胞系（ＬＡ－７９５）高、低转移亚系（ＬＡ－７９５－Ｃ＿６和ＬＡ－７９５－Ｃ＿７）体外培养第２０及３０代细胞染色体Ｇ显带核型进行比较，结果高转移亚系细胞染色体众数较低转移亚系细胞具有不稳定性的特点，表明转移潜能的差异和遗传物质的稳定程度密切相关．高、低转移亚系出现相同及不同的标志染色体和异常染色体，其中，高转移亚系出现标志染色体８种及ｄｅｌ（８）ｑ￣（ｔｅｒ），ｄｅｌ（１２）ｑ￣（ｔｅｒ），１１Ｐ￣＋，１３Ｐ￣＋，ｔｒｉ（２Ｍ４），ｄｉｃ（２Ｍ５），Ｍ６：７ｑ￣＋？；而低转移潜能亚系出现标志染色体１２种及ｄｅｌ（８）ｑ￣（ｔｅｒ），ｄｅｌ（１２）ｑ￣（ｔｅｒ），ｒ（１５），ｄｉｃ（Ｍ６；２Ｍ７），Ｍ８：７ｑ￣＿？ｔ（２：２），ｔ（２：１５），ｔ（１５：Ｍ１０），ｔ（１５：Ｍ１１），ｔ（５：Ｍ１２）．表明来源于同一母系而转移潜能不同的亚系染色体核型具有本质上的差异，转移潜能与其密切相关．另外，分析低转移亚系出现ｔ（２：２）；ｔ（Ｃ２：１５）ｔ（１５：Ｍ１０）；ｔ（１５：Ｍ１１）；　ｔ（５：Ｍ１２）；ｒ（１５）等现象，推测高、低转移潜能亚系细胞可能存在本身Ｈ－２系统及ＣＤ４４抗元表达的差异，这很可能     

原发性肝癌P53基因第249密码子突变的研究

用聚合酶链反应－限制性片段长度多态性（ＰＣＲ－ＲＦＬＰ）技术对７例原发性肝细胞癌（ＰＨＣ）进行Ｐ＾５３基因第２４９密码子突变的检测。结果：７例肝细胞癌中３例有Ｐ＾５３基因第２４９密码子突变，突变率达４２．９％。     

中国人P型铜转运ATP酶基因突变的初步研究

应用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）及测序等方法分析了１４１便ＷＤ患者ＡＴＰ７Ｂ基因第７、９及１４外显子的部分ＤＮＡ序列改变，通过对患者ＤＮＡＴ下沉人ＤＮＡＲＰＣＲ－ＳＳＣＰ电泳带迁移作对比分析，发现在１４１例患者中第７、９及１４外显子ＰＣＲ扩增产物迁移异常才分别为４例、６例和４０例，依据ＤＮＡ物单链构聚与分子电泳迁移的关系，提示该组病人中ＡＴＰ７Ｂ基因第７、９和１４外显子的突变率     

新型手性衍生化试剂MTPEA^13C NMR的研究

本文通过质子宽带去耦（ＰＢＢＤ）和质子偏共振去耦（ＰＯＲＤ）核磁共振技术；研究了新型手性衍生化试剂２－甲氧基－２－三氟甲基－２－苯基－乙胺（ＭＴＰＥＡ）分子中＾１３Ｃ－＾１Ｈ、＾１３Ｃ－＾１２Ｆ间的耦合作用。在此基础上，报导了ＭＴＰＥＡ分子中各基团的化学位移和＾１３Ｃ－＾１９Ｆ间的一级、二级耦合常数（＾１Ｊ和＾２Ｊ）。     

β—内酰胺酶基因在谷氨酸产生菌T6—13染色体上的…

以质粒ｐＵＣ１３编码的β－内酰胺酶基因为外源基因，通过随机连接谷氨酸产生菌Ｔ６－１３染色体ＤＮＡ片段后转化Ｔ６－１３原生质体，使其整合到染色体上，得以表达。在实验条件下，所测１９株转化子的抗性都有很高的稳定性，其中１０株能百分之百地保持抗性。经生物素标记的ｐＵＣ１３为探针的分子杂交实验证实抗性基因已整合到Ｔ６－１３菌染色体ＤＮＡ上。     

Ramsey数R（K3,Kq—e）^1）

利用一种系统地构造循环着的算法,借助计算机证明了Ｒａｍｓｅｙ数Ｒ（Ｋ３,Ｋｑ－ｅ）的下述新下界：Ｒ（Ｋ３,Ｋ１１－ｅ）≥４２,Ｒ（Ｋ３Ｋ１３－ｅ）≥５４,Ｒ（Ｋ３,Ｋ１４－ｅ）≥５９,Ｒ（Ｋ３,Ｋ１５－ｅ）≥６９。     

核反应在谷物蛋白质测量中的应用

利用１３ＭｅＶ氘束在＾１４Ｎ和＾１２Ｃ内引发（ｄ，Ｐ）反应测量了小麦和大米的＾１４Ｎ／＾１２Ｃ比率，讨论了在农作物繁殖方面利用该技术的可能性。     

Clustering of breakpoints on chromosome 11 in human B-cell neoplasms with the t(11;14) chromosome translocation

The t(11;14) (q13;q32) chromosome translocation has been reported in diffuse small and large cell lymphomas and in chronic lymphocytic leukaemia (B-CLL) and multiple myeloma. Because chromosome band 14q32 is involved in this translocation, as well as in the t(8;14) (q24;q32) translocation of the Burkitt tumour, interruption of the immunoglobulin heavy-chain locus was postulated for this rearrangement. We have cloned the chromosomal joinings between chromosomes 11 and 14 and also between chromosomes 14 and 18, in B-cell tumours carrying translocations involving these chromosomes, and suggested the existence of two translocated loci, bcl-1 and bcl-2, normally located on chromosomes 11 (band q13) and 18 (band q21) respectively, involved in the pathogenesis of human B-cell neoplasms. The results indicate that in the leukaemic cells from two different cases of CLL, the breakpoints on chromosome 11 are within 8 nucleotides of each other and on chromosome 14 involve the J4-DNA segment. Because we detected a 7mer-9mer signal-like sequence with a 12-base-long spacer on the normal chromosome 11, close to the breakpoint, we speculate that the t(11;14) chromosome translocation in CLL may be sequence specific and may involve the recombination system for immunoglobulin gene segment (V-D-J) joining.     

Genetic imprinting suggested by maternal heterodisomy in nondeletion Prader-Willi syndrome

Prader-Willi syndrome (PWS) is the most common form of dysmorphic genetic obesity associated with mental retardation. About 60% of cases have a cytological deletion of chromosome 15q11q13 (refs 2, 3). These deletions occur de novo exclusively on the paternal chromosome. By contrast, Angelman syndrome (AS) is a very different clinical disorder and is also associated with deletions of region 15q11q13 (refs 6-8), indistinguishable from those in PWS except that they occur de novo on the maternal chromosome. The parental origin of the affected chromosomes 15 in these disorders could, therefore, be a contributory factor in determining their clinical phenotypes. We have now used cloned DNA markers specific for the 15q11q13 subregion to determine the parental origin of chromosome 15 in PWS individuals not having cytogenetic deletions; these individuals account for almost all of the remaining 40% of PWS cases. Probands in two families displayed maternal uniparental disomy for chromosome 15q11q13. This is the first demonstration that maternal heterodisomy--the presence of two different chromosome 15s derived from the mother--can be associated with a human genetic disease. The absence of a paternal contribution of genes in region 15q11q13, as found in PWS deletion cases, rather than a mutation in a specific gene(s) in this region may result in expression of the clinical phenotype. Thus, we conclude that a gene or genes in region 15q11q13 must be inherited from each parent for normal human development.     

Correlation between immunoglobulin light chain expression and variant translocation in Burkitt's lymphoma

Burkitt's-type lymphomas-leukaemias (BL) are monoclonal proliferations of malignant B lymphocytes. Irrespective of whether they carry the Epstein-Barr virus (EBV) genome, these tumour cells have been shown consistently to have one of the specific reciprocal chromosome translocations, t(8; 14), t(2; 8) or t(8; 22), involving the long arm of chromosome 8 (on 8q24) and chromosome 14, 2 or 22 (on 14q32, 2p12 and 22q11, respectively). The latter chromosomes have been shown recently to carry genes for immunoglobulin (Ig) heavy chains, and kappa and lambda light chains, respectively. Furthermore, the localization of kappa light chains within 2pcen-2p13 encompasses the breakpoint observed in Burkitt's translocation (2p12). It was therefore considered of interest to determine whether the expression of immunoglobulin chains in BL cells is related to the type of chromosomal anomalies observed. We report here that there is a direct relationship between expression of immunoglobulin light chains and specific type of translocation: BL cells with t(8; 22) express lambda chains, whereas those with t(2; 8) express kappa chains.     

外源hDAF基因对转基因猪影响的初步研究

应用显微注射方法将外源hDNA基因导入湖北白猪的受精卵,获得了相应的转基因猪,4．2,8．0kb两种导入外源基因片断移植后死胎率分别为20．00％,39．71％,差异不显著,8．0kb基因产仔畸形率为11．76％,而4．3kb基因无畸形,结果表明,原核基因对转基因猪有明显的致畸作用,G0代转基因猪60日龄重,6月龄重对照组差异均不显著,表明外源基因的整合位点较理想。     

A chromosome 14 inversion in a T-cell lymphoma is caused by site-specific recombination between immunoglobulin and T-cell receptor loci

Specific chromosomal aberrations are associated with specific types of cancer (for review see ref. 1). The distinctiveness of each association has led to the belief that these chromosomal aberrations are clues to oncogenic events or to the state of differentiation in the malignant cell type. Malignancies of T lymphocytes demonstrate such an association characterized most frequently by structural translocations or inversions of chromosomes 7 and 14 (refs 7-9). Analyses of these chromosomally marked tumours at the molecular level may therefore provide insight into the aetiology of the cancers as well as the mechanisms by which chromosomes break and rejoin. Here we report such an analysis of the tumour cell line SUP-T1 derived from a patient with childhood T-cell lymphoma carrying an inversion of one chromosome 14 between bands q11.2 and q32.3, that is, inv(14) (q11.2; q32.2). These are the same chromosomal bands to which the T-cell receptor alpha-chain (14q11.2) and the immunoglobulin heavy-chain locus (14q32.3) have been assigned. Our analysis reveals that this morphological inversion of chromosome 14 was mediated by a site-specific recombination event between an immunoglobulin heavy-chain variable region (Ig VH) and a T-cell receptor (TCR) alpha-chain joining segment (TCR J alpha). S1 nuclease analysis shows that this hybrid gene is transcribed into poly(A)+ RNA.     

小鼠转基因及传代研究

综述了湖北省农科院生物技术研究所近年来有关转基因小鼠的主要研究进展。应用原核注射技术 ,将POMT PGH、hDAF、pBHSA、pSHSA、PT HBV 1 3、Bcl xL、hCD5 9、hCD5 9+hMCP等 8种外源基因 ,注入并移植 4 874枚小鼠的受精卵 ,得到 5 5 6只小鼠 ;经PCR和Southern杂交检测 ,确认原代转基因小鼠 10 8只。基因整合率平均 19.4 % ,转基因效率平均 2 .2 %。应用混合注射的方法得到了转双基因小鼠 ,双基因共整合率 2 2 .2 %。表达外源蛋白的转基因小鼠在 5 0 %～ 10 0 %之间。研究了小鼠的超数排卵和影响转基因效率的几个因素。通过转Bcl xL小鼠与非转基因小鼠连续五个世代的传代交配和检测 ,研究了转基因小鼠外源基因的遗传规律 ,表明四只原代小鼠中 ,只有一只能稳定地将外源基因传递给后代。并非所有的转基因小鼠都具有遗传的稳定性 ,欲建立小鼠的转基因品系 ,尚需对原代转基因小鼠进行筛选。     

南京产日本林蛙染色体组型

本文报导了用骨髓细胞法对南京产日本林蛙的染色体组型进行研究的结果,並将其与国内外有关资料进行了对比。     

Probing the Proteolysis of Melitin Using Liquid Secondary Ion Mass Spectrometry

ＰｒｏｂｉｎｇｔｈｅＰｒｏｔｅｏｌｙｓｉｓｏｆＭｅｌｉｔｉｎＵｓｉｎｇＬｉｑｕｉｄＳｅｃｏｎｄａｒｙＩｏｎＭａｓｓＳｐｅｃｔｒｏｍｅｔｒｙ＊ＷｕＹｉ＋（武轶），ＷａｎｇＪｉｎｇｚｕｎ（王敬尊），ＳｕｉＳｅｎｆａｎｇ＋（隋森芳）＊＋Ｄｅｐａｒｔｍｅ...     

FISH analysis of the integration patterns in transgenic rice co-transformed by microprojectile bombardment

Using multi-color fluorescencein situ hybridization (FISH), we localized transferredbarnase-ps1 andpHctinG DNA sequences onto chromosomes of two transgenic rice plants, named Q12 and Q13, both of which were produced by micro-projectile bombardment. In both Q12 and Q13, each detected cell showed 2–3 signal spots on their chromosomes respectively. The signals of bothbarnase-ps1 andpHctinG were mostly detected in the adjacent chromosomal sites in which their signals were overlapped and could be recognized by the signal color on the metaphase chromosomes. Fiber FISH further demonstrated that the multiple copies in each of the two DNA sequences distributed adjacently on the DNA fiber in Q13. Combined with the results of Southern hybridization, the possible integration patterns in transgenic rice co-transformed by micro-projectile bombardment have been discussed.     

Breakpoints in the human T-cell antigen receptor alpha-chain locus in two T-cell leukaemia patients with chromosomal translocations

Specific chromosomal translocations have been observed in several human and animal tumours and are believed to be important in tumorigenesis. In many of these translocations the breakpoints lie near cellular homologues of transforming genes, suggesting that tumour development is partly due to the activation of these genes. The best-characterized example of such a translocation occurs in mouse plasmacytoma and human B-cell lymphoma, where c-myc, the cellular homologue of the viral oncogene myc, is brought into close proximity with either the light- or heavy-chain genes of the immunoglobulin loci, resulting in a change in the regulation of the myc gene. T-cell malignancies also have characteristic chromosomal abnormalities, many of which seem to involve the 14q11-14q13 region. This region has recently been found to contain the alpha-chain genes of the human T-cell antigen receptor. Here we determine more precisely the chromosome breakpoints in two patients whose leukaemic T cells contain reciprocal translocations between 11p13 and 14q13. Segregation analysis of somatic cell hybrids demonstrates that in both patients the breakpoints occur between the variable (V) and constant (C) region genes of the T-cell receptor alpha-chain locus, resulting in the translocation of the C-region gene from chromosome 14 to chromosome 11. As the 11p13 locus has been implicated in the development of Wilms' tumour, it is possible that either the Wilms' tumour gene or a yet unidentified gene in this region is involved in tumorigenesis and is altered as a result of its translocation into the T-cell receptor alpha-chain locus.