| 磁场对纤维素酶的活性及构象的影响 |
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| 引用本文： | 贺华君,朱元保,钟科军,曹祉祥,何双娥.磁场对纤维素酶的活性及构象的影响[J].吉首大学学报(自然科学版),1998(1). |
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| 作者姓名： | 贺华君 朱元保 钟科军 曹祉祥 何双娥 |
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| 作者单位： | 湖南大学化学化工学院!长沙，410082，华东理工大学生物化工学院，上海，200237，湖南大学化学化工学院!长沙，410082，湖南大学化学化工学院!长沙，410082，湖南大学化学化工学院!长沙，410082，湖南大学化学化工学院!长沙，410082 |
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| 摘 要： | 以羧甲基纤维素钠（CMC）为底物评价纤维素酶的活性，研究了不同条件下静磁场对酶的活性及构象的影响。9℃，pH4．0条件下磁化，其活性及荧光光谱均不见明显变化；但若改变反应时的pH，可使活性增加达16.4％；并发现磁处理后的酶有滞后效应及可逆性。较高温度下，磁化处理酶液，其活性及构橡均发生不同程度的变化，且对其抑制动力学表现为混合型抑制模式；其构象变化也基本上随失活的增加荧光强度增大。
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| 关 键 词： | 磁场 纤维素酶 催化活性 构象 |
Effect of Magnetic Field on Cellulase Activity and Conformation |
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| He Huajun, Zhu Yuanbao, Zhong Kejun, Chao Zhiqiang, He Shuange.Effect of Magnetic Field on Cellulase Activity and Conformation[J].Journal of Jishou University(Natural Science Edition),1998(1). |
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| Authors: | He Huajun Zhu Yuanbao Zhong Kejun Chao Zhiqiang He Shuange |
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| Abstract: | Cellulase actlvity is measured on sodium carboxymethyl -cellulose substrate by Dinitrosalicylic acid(DNS) method. No obvious effect is observed when magnetic field on cellulase at 9℃,pH4.0. Once change the reaction pH, the activity will be increased by 16. 4%,the delaying and reversible effect is also observed- At 60℃, the effect on the activity and conformation changes at different extent. A Lineweaver-Burk plot of the reaction shows the typical pattern of mixed-type inhibition. It seems that the intensity of the fluorescence increases with the decrease of the activity. |
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| Keywords: | magnetic field cellulase catalytic activity conformation |
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