| 生物膜活性测定中TTC-脱氢酶活性测定法的改进 |
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| 引用本文： | 李今,吴振斌,贺锋.生物膜活性测定中TTC-脱氢酶活性测定法的改进[J].吉首大学学报(自然科学版),2005,26(1):37-39. |
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| 作者姓名： | 李今 吴振斌 贺锋 |
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| 作者单位： | (1.中国科学院水生生物研究所淡水生态和生物技术国家重点实验室，湖北 武汉 430072；2.中国科学院研究生院，北京100039；3.湖北师范学院生物系，湖北 黄石 435002) |
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| 基金项目： | 国家"十五"重大科技专项资助项目(2002AA601021)，湖北省教育厅重点资助项目(2002A00010) |
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| 摘 要： | 对生物膜活性测定中氯化三苯基四氮唑(TTC)-脱氢酶活性测定法进行了改进，解决了标准曲线制作稳定性差的问题，对测定中的诸多影响因素进行比较分析，确定了改进后的脱氢酶活性测定的最佳条件.结果表明，以甲苯作为萃取剂的液-液分层明显，提取效果好，操作简便.以硫化钠代替连二亚硫酸钠作还原剂，效果较好，显色稳定不褪色.反应的适宜pH值为8.6，适宜温度为38 ℃.同时确定了生物膜的最佳培养反应时间为6 h.
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| 关 键 词： | 生物膜 TTC 脱氢酶活性 标准曲线 |
| 文章编号： | 1007-2985(2005)01-0037-03 |
| 修稿时间： | 2004年12月11 |
Improvement of Determination on TTC-Dehydrogenase Activity in Biofilms |
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| LI Jin,WU Zhen-bin,HE Feng.Improvement of Determination on TTC-Dehydrogenase Activity in Biofilms[J].Journal of Jishou University(Natural Science Edition),2005,26(1):37-39. |
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| Authors: | LI Jin WU Zhen-bin HE Feng |
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| Institution: | (1.State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,Hubei China；2.Graduate School of the Chinese Academy of Sciences,Beijing 100039;3.Biology Department,Hubei Normal University,Huangshi 435002,Hubei China) |
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| Abstract: | The method of 3,5,5-triphenyltetrazolium (TTC) determining biofilms activity was improved,and the problem that the standard curve of determination on TTC-dehydrogenase activity can’t be made conveniently and stably was resolved.The factors which influence the determination were studied.The results showed that sodium sulfide as reducer,toluene as TF organic extractant,Tris-HCl as buffer solution,pH 8.6 and 38 ℃ were the best condition on the developed method,and the optimum reaction time to cultivate biofilms is 6 h. |
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| Keywords: | biofilms TTC dehydrogenase activity standard curve |
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