月见草花黄色素提取工艺研究

对月见草花黄色素的提取条件进行了系统的研究，比较了无水乙醇、95％乙醇、乙醚、丙酮、乙酸乙酯、石油醚提取月见草花黄色素的效果.结果显示：以95％乙醇为最佳溶剂，95％乙醇提取液的λmax为394 nm.单因素试验和正交试验表明，温度对提取月见草花黄色素的影响较大，而浸提时间和料液比影响较小.提取的最佳工艺条件是：以体积分数95％的乙醇为浸提剂，按每0.2 g月见草花粉末加浸提剂30 mL的比例投料，在温度60 ℃下，浸提时间为4 h.月

Study on Extraction Technology of Yellow Pigment From Oenothera Brennis L.Flower

The extraction conditions of yellow pigment from Oenothera brennis.L flower were studied.The extraction effects by pure ethanol,95％ alcohol,ether,acetone,ethyl ethanoate,petroleum benzene were compared,and the result indicated that 95％ alcohol was the optimum solvent.the maximum absorption wavelength(λmax) of the 95％ alcohol being 394 nm.Mono-factor experiments and orthogonal experiments showed that the experiment temperature could have a greater impact on extraction of the yellow pigment than the ratio of the solvent to petal and extraction time of solvent.The result showed that the optimum conditions are the following:the extraction reagent 95％ alcohol,the petal and 95％ alcohol  were put in container with the ratio of 0.2∶30(g/mL) then heated at 60 ℃ for 4 h.