| 水稻苯达松敏感致死基因的RAPD标记的克隆及测序 |
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| 引用本文： | 彭凌,朱必凤,刘主,刘盈盈.水稻苯达松敏感致死基因的RAPD标记的克隆及测序[J].韶关学院学报,2005,26(12):78-81. |
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| 作者姓名： | 彭凌 朱必凤 刘主 刘盈盈 |
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| 作者单位： | 1. 韶关学院,英东生物工程学院,广东,韶关,512005
2. 南昌大学,生命科学学院,江西,南昌,330047 |
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| 基金项目： | 广东省自然科学基金资助项目（001446） |
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| 摘 要： | 用3S Spin DNA Agarose Gel Purification Kit试剂盒将与水稻苯达松敏感致死基因相连锁的RAPD遗传标记S20—420和S316—600回收纯化，连接于pGEM—T载体并克隆测序，得到了S20—420和S316—600的全序列，其长度分别为423bp、606bp．将两端序列设计特异PCR扩增引物可用于检测水稻苯达松敏感致死基因和标记辅助育种．
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| 关 键 词： | 水稻 苯达松敏感致死基因 RAPD 克隆 测序 |
| 文章编号： | 1007-5348(2005)12-0078-04 |
| 收稿时间： | 08 29 2005 12:00AM |
| 修稿时间： | 2005年8月29日 |
Cloning and Sequence of RAPD Marker Linked to the Bentazon Susceptible Lethality Gene in Rice |
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| PENG Ling,ZHU Bi-feng,LIU Zhu,LIU Ying-ying.Cloning and Sequence of RAPD Marker Linked to the Bentazon Susceptible Lethality Gene in Rice[J].Journal of Shaoguan University(Social Science Edition),2005,26(12):78-81. |
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| Authors: | PENG Ling ZHU Bi-feng LIU Zhu LIU Ying-ying |
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| Institution: | 1. Yingdong College of Bioengineering, Shaoguan University, Shaoguan 512005, Guangdong, China; 2.Life Science College, Nanehang University, Nanehang 330047, Jiangxi, China |
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| Abstract: | Randomly amplified polymorphic DNA(RAPD) was employed to detect molecular markers linked to the bentazon susceptible lethality gene in rice.RAPD marker S20-420 and S316-600 were linked to the bentazon susceptible lethality gene in rice.S20-420 and S316-600 fragment were reclaimed by 3S Spin DNA Agarose Gel Purification Kit and cloned in T-easy vector.Sequencing finally showed that the S20-420 fragment was actually 423 bp in lenth,the S316-600 fragment was actually 606 bp.It is suggested that the sequence of RAPD markers might be used as a basis for synthesizing the specific PCR primers and for detecting the bentazon susceptible lethality gene in rice and molecular marker assisted selection(MAS). |
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| Keywords: | rice bentazon susceptible lethality gene RAPD cloning sequence |
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