REGγ,p53,NOTCH1和BRAF在甲状腺癌中的表达及其临床意义

旨在探讨REGγ,p53,NOTCH1和BRAF在乳头状甲状腺癌中的表达及其与临床病理因素的相关性.应用Realtime-PCR及免疫组织化学方法检测REGγ,p53,NOTCH1和BRAF在乳头状甲状腺癌组织中的表达,分析REGγ与p53,NOTCH1,BRAF之间的关系及其与临床病理因素的相关性.REGγ在乳头状甲状腺癌组织中表达明显增高,且其阳性率为87.5％(35/40).REGγ表达与患者年龄、淋巴结转移情况有关(P=0.015 1＜0.05),与患者性别无显著性相关(P=0.742＞0.05);REGγ表达与p53,NOTCH1和BRAF表达呈正相关(P=0.023 3＜0.05).REGγ阳性表达可作为乳头状甲状腺癌发生、发展的生物学指标,联合p53、NOTCH1和BRAF检测可以为临床诊治及预后判断提供参考.

Expressions of REG_γ,p53,NOTCH1and BRAF gene in thyroid cancer and their clinical relevance

This study was designed to investigate the expressions of〖WTBX〗 REGγ〖WTBZ〗,〖WTBX〗 〖STBX〗p53〖WTBZ〗, 〖WTBX〗NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in papillary thyroid cancer as well as the potential correlations with clinicopathological significance. The expressions of 〖WTBX〗REGγ〖WTBZ〗,〖WTBX〗 p53〖WTBZ〗,〖WTBX〗 NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 in thyroid cancer samples were detected by Realtime-PCR and immunohistochemistry, respectively. The correlations with clinicopathological factors were statistically analyzed. The expression of 〖WTBX〗REGγ〖WTBZ〗 in papillary thyroid cancer samples was significantly higher, with an 87.5% (35/40) positive rate in cancer tissues, and was correlated with age and lymph node status (〖WTBX〗P〖WTBZ〗=0.015 1<0.05), but not related to sex (〖WTBX〗P〖WTBZ〗=0.742>0.05). In addition, the 〖WTBX〗REGγ〖WTBZ〗 had a positive correlation with the levels of 〖WTBX〗〖STBX〗p53, NOTCH1〖STBZ〗〖WTBZ〗 and 〖WTBX〗BRAF〖WTBZ〗 (〖WTBX〗P〖WTBZ〗=0.023 3<0.05), indicating that 〖WTBX〗REGγ〖WTBZ〗 could serve as an objective biological-marker for estimating the occurrence and progression of papillary thyroid cancer. Combined detection of 〖WTBX〗〖STBX〗p53〖WTBZ〗,〖WTBX〗 NOTCH1〖WTBZ〗 and 〖WTBX〗BRAF 〖WTBZ〗may facilitate clinical diagnosis, treatment and prediction of prognosis.