| 电磁脉冲各参数对HeLa细胞可逆穿孔影响的研究 |
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| 引用本文： | 杨孔,王子淑,郭聪,邹方东,王喜忠,王保义,张弘,刘长军.电磁脉冲各参数对HeLa细胞可逆穿孔影响的研究[J].四川大学学报(自然科学版),2001,38(4):575-579. |
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| 作者姓名： | 杨孔 王子淑 郭聪 邹方东 王喜忠 王保义 张弘 刘长军 |
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| 作者单位： | 1. 四川大学生命科学学院,
2. 四川大学无线电系, |
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| 基金项目： | 国家自然科学基金(60071024) |
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| 摘 要： | 通过对HeLa细胞进行电磁脉冲处理,可以看到细胞膜电穿孔.穿孔的几率及深度与脉冲的各参数有关.细胞膜表面的微绒毛受到一定程度的损伤,还可见到火山形的喷口和喷出物.经实验研究,得到了在离体条件下HeLa细胞电穿孔的最佳电脉冲参数.
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| 关 键 词： | HeLa细胞 电磁脉冲 电穿孔 电化学治疗 |
| 文章编号： | 0490-6756(2001)04-0575-05 |
| 修稿时间： | 2001年3月9日 |
STUDY OF THE EFFECT OF REVERSIBLE PORATION OF HELA CELL HANDLED BY EMPF WITH VARIOUS PARAMETERS |
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| YANG kong ,WANG Zi shu ,GUO Cong ,ZHOU Fang dong ,WANG Xi zhong ,WANG Bao yi ,ZHANG Hong ,LIU Chang jun.STUDY OF THE EFFECT OF REVERSIBLE PORATION OF HELA CELL HANDLED BY EMPF WITH VARIOUS PARAMETERS[J].Journal of Sichuan University (Natural Science Edition),2001,38(4):575-579. |
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| Authors: | YANG kong WANG Zi shu GUO Cong ZHOU Fang dong WANG Xi zhong WANG Bao yi ZHANG Hong LIU Chang jun |
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| Institution: | YANG kong 1,WANG Zi shu 1,GUO Cong 1,ZHOU Fang dong 1,WANG Xi zhong 1,WANG Bao yi 2,ZHANG Hong 2,LIU Chang jun 2 |
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| Abstract: | When HeLa cell handled by EMPT with various voltages,times,capacitance is contrast put in with unhandled cell,the handled cell is found to have been electropored.The frequence,pores number,depth of poration are relative to the EMPF parameters. At the same time,the microvilli is damaged.Moreover, the autheors have also seen the pore like of volcanic vent and ejecta.The authors have found the best parameters of EMPF that handled HeLa cell. All above provides primary evidence for the electrochemotherapy of HeLa cell. |
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| Keywords: | HeLa cell EMPF electroporation electrochemotherapy |
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