| 盐藻GPDH基因的载体构建及在烟草中的表达 |
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| 引用本文： | 张华,乔代蓉,白林含,张庆莲,邓雪柯,曹毅.盐藻GPDH基因的载体构建及在烟草中的表达[J].四川大学学报(自然科学版),2004,41(2):436-439. |
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| 作者姓名： | 张华 乔代蓉 白林含 张庆莲 邓雪柯 曹毅 |
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| 作者单位： | 四川大学生命科学学院,成都,610064 |
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| 基金项目： | 国家863转基因项目(2002AA213021)，国家自然科学基金(30270711) |
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| 摘 要： | 作者用冻融法，将构建有盐藻3-磷酸甘油脱氢酶基因的植物高效表达载体DsGPDH导入感受态的根癌农杆茵EHA105中．叶盘法转化烟草，在含卡那霉素的MS培养基上进行选择性培养，生根率达到80％．用POR的方法对再生苗进行了初步筛选，阳性率约为50％．对PCR阳性苗，进行RT-PCR分析，证实整合到烟草基因组中的GPDH基因表达产生了mRNA．
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| 关 键 词： | 盐藻 GPDH基因 叶盘转化法 转基因烟草 |
| 文章编号： | 0490-6756(2004)02-0436-04 |
Vector Construction and Expression in Tobacco of GPDH Gene from Dunaliella salina |
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| ZHANG Hua,QIAO Dai-rong,BAI Lin-han,ZHANG Qing-lian,DENG Xue-ke,CAO Yi.Vector Construction and Expression in Tobacco of GPDH Gene from Dunaliella salina[J].Journal of Sichuan University (Natural Science Edition),2004,41(2):436-439. |
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| Authors: | ZHANG Hua QIAO Dai-rong BAI Lin-han ZHANG Qing-lian DENG Xue-ke CAO Yi |
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| Abstract: | The plant transformation vector DsGPDH,which was constructed to express the glycerol-3-phosphate dehydrogenase gene was introduced into competent Agrobacterium tumefaciens EHA105 using the freeze-thaw method.After infected by A.tumefaciens,the leaf discs of tobacco were selected on plates containing MS inducement medium supplemented with 100mg/L kanamycin,the rate of rootage was 80%.The regenerated plants were identified.About 50% of tested plants produced the target band by PCR.RT-PCR analysis showed that GPDH gene integrated into tobacca genome has produced mRNA. |
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| Keywords: | GPDH gene Dunaliella salina leaf discs transformation transgenic tobacco |
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