| 两类微生物SOD的比较研究 |
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| 引用本文： | 王忠彦,黄英.两类微生物SOD的比较研究[J].四川大学学报(自然科学版),1996,33(6):733-738. |
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| 作者姓名： | 王忠彦 黄英 |
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| 摘 要： | 报道了酵母ＳＯＤ工程菌ＺＨ－１／ｐＳＨ１比耐高温细菌Ｂ．Ｓ２１１－１５的抗Ｈ２Ｏ２水平高，其单位体积培养液和酵得率约为细菌的二倍，但单位细胞酶的含量比细菌低，高温细菌的ＳＯＤ热稳定性和存放稳定比酵母工程菌好，二者在ｐＨ４－１０范围内都很稳定，但对蛋白的耐受性差异很大，其中酵母ＳＯＤ工程勤务产好。
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| 关 键 词： | 酵母 细菌 超氧化物歧化酶 |
COMPARISONS OF THE SOD OF TWO KINDS OF MICROORGANISM |
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| Wang,Zhongyan Huang,Ying Men Yun Hu,Yongsong.COMPARISONS OF THE SOD OF TWO KINDS OF MICROORGANISM[J].Journal of Sichuan University (Natural Science Edition),1996,33(6):733-738. |
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| Authors: | Wang Zhongyan Huang Ying Men Yun Hu Yongsong |
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| Institution: | Department of Biotechnology |
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| Abstract: | The H 2O 2 resistence of SOD engineering yeast was higher than that of thermophilric bacterium.The per volum SOD production of the yeast was higher than that of the bacterium whose SOD content was however higher.The heat and storing stabilities of the SOD of the bacterium were better than those of the yeast.They both had good pH stability,but their protease tolerance were much different.The results of inhibition reactions showed that the SOD of the bacterium was Fe SOD,while that of the yeast was mainly Cu/Zn SOD with a little Mn SOD.They all showed active bonds of dimer or tetramer in PAGE. |
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| Keywords: | yeast bacterium superoxide dismutase (SOD) |
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