| 正丙醇、铜离子、烟酰胺对红霉素合成的作用 |
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| 引用本文： | 刘勇,叶蕊芳,郑黎,李晓勇,韩素芳.正丙醇、铜离子、烟酰胺对红霉素合成的作用[J].华东理工大学学报(自然科学版),2005,31(6):808-811. |
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| 作者姓名： | 刘勇 叶蕊芳 郑黎 李晓勇 韩素芳 |
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| 作者单位： | 华东理工大学生物反应器工程国家重点实验室,上海,200237;河南天方药业有限公司,河南,驻马店,463600 |
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| 摘 要： | 在摇瓶培养过程中，发酵初期加入正丙醇可使红霉素的效价提高49．31％，正丙醇提高了丙酸激酶的比活力，表现出对该酶诱导作用，而对丙酰CoA合成酶比活力无影响。加入铜离子和烟酰胺可使红霉素效价分别提高24％和179／5。用HPLC测定发酵液中有机酸含量，分析测定结果认为发酵过程可分为3个阶段：积累期、转折期和生产期。
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| 关 键 词： | 红霉素 正丙醇 烟酰胺 铜离子 合成 |
| 文章编号： | 1006-3080(2005)06-0808-04 |
| 收稿时间： | 2004-11-28 |
| 修稿时间： | 2004年11月28 |
Effects of Propanol, Cu2+ and Niacinamide on Biosynthesis of Erythromycin |
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| LIU Yong,YE Rui-fang,ZHENG Li,LI Xiao-yong,HAN Su-fang.Effects of Propanol, Cu2+ and Niacinamide on Biosynthesis of Erythromycin[J].Journal of East China University of Science and Technology,2005,31(6):808-811. |
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| Authors: | LIU Yong YE Rui-fang ZHENG Li LI Xiao-yong HAN Su-fang |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China ; 2. Henan Top fond Pharmaceutical Co. Ltd. , Henan Zhumadian 463600, China |
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| Abstract: | Addition of propanol during erythromycin fermentation with in shake flask improved the titer by 49.3%.Propanol was found to increase the specific activity of propionate kinase,but it had no(influence) on the specific activity of propionyl-CoA synthetase.Addition of Cu~(2+) and niacinamide,(increased erythromycin) titer by 24% and 17%,respectively.Using HPLC,organic acid in the broth were measured;it was suggested that the fermentation process can be divided into three phases: accumulative phase,transitional phase and production phase.In production phase,the productivity of erythromycin was the highest. |
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| Keywords: | erythromycin propanol propionyl-CoA synthetase Cu~(2+) synthesis |
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