| 静息培养基的筛选及在此体系中庆大霉素的合成 |
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| 引用本文： | 谌颉,储炬,张嗣良,李友荣.静息培养基的筛选及在此体系中庆大霉素的合成[J].华东理工大学学报(自然科学版),2001,27(2):135-138. |
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| 作者姓名： | 谌颉 储炬 张嗣良 李友荣 |
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| 作者单位： | 华东理工大学 |
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| 基金项目： | 国家自然科学基金资助项目 !(3 9870 0 18) |
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| 摘 要： | 通过对比实验，讨论了培养基中各种组分对庆大霉素合成与分泌的影响；由此确定了静息细胞培养基的配比，并应用此系统考察了影响庆大霉素合成的因素。结果表明：培养温度为34℃、添加适量次级代谢物、体系内硫酸锰浓度为500mg／L及每升添加0．020mL的Tween20都肥促进庆大霉素的合成。
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| 关 键 词： | 庆大霉素 静息细胞 培养基 筛选 合成 |
| 文章编号： | 1006-3080(2001)02-0135-04 |
| 修稿时间： | 2000年5月3日 |
Screening Resting Cell System for Study Gentamicin Biosynthesis |
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| CHEN Jie,Chu Ju,ZHANG Si-Liang,LI You-rong.Screening Resting Cell System for Study Gentamicin Biosynthesis[J].Journal of East China University of Science and Technology,2001,27(2):135-138. |
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| Authors: | CHEN Jie Chu Ju ZHANG Si-Liang LI You-rong |
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| Abstract: | Through comparative experiments, the concentration of each ingredient in the resting cell medium was determined, and their influences on the synthesis and secretion of gentamicin(GM) were also discussed. Some factors affecting gentamicin biosynthesis were studied by using this system. The results showed that the biosynthesis of GM was promoted when the temperature was controlled at 34°C and some secondary metabolites, 50mg/L MnSO4 or 0.02mL Tween20 per liter were supplemented. |
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| Keywords: | gentamicin resting cell culture medium screening synthesis |
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