| 赤子爱胜蚓(Eisenia Foelide)纤维素酶的初步研究 |
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| 引用本文： | 严民宏,王二力,周元聪,陈远聪,陶宗晋.赤子爱胜蚓(Eisenia Foelide)纤维素酶的初步研究[J].华东理工大学学报(自然科学版),1991(1). |
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| 作者姓名： | 严民宏 王二力 周元聪 陈远聪 陶宗晋 |
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| 作者单位： | 华东化工学院生物工程系
(严民宏，王二力)，中国科学院上海生物化学研究所
(周元聪，陈远聪)，中国科学院上海脑研究所(陶宗晋) |
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| 摘 要： | 将赤子爱胜蚓整体组织匀浆的抽提液,经Sephadex G-75凝胶过滤、DEAE-Sepharose CL-6B离子交换及在FPLC仪上用Mono Q柱分离,可以纯化到多种组分的纤维素酶,研究了它们的酶学性质,证明均为C_X酶。
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| 关 键 词： | 纤维素酶 蚯蚓 C_1酶 C_x真酶 酶的纯化 |
Primary Studies of Cellulase from Earthworm (Eisenia Foelide) |
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| Yan Minhong,Wang Erli.Primary Studies of Cellulase from Earthworm (Eisenia Foelide)[J].Journal of East China University of Science and Technology,1991(1). |
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| Authors: | Yan Minhong Wang Erli |
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| Abstract: | Multiple forms of cellulase were isolated and purified from earthworm (Eisenia Foelide) homogenate by Sephadex G~*75 gel filtration, ion exchange chromatography on DEAE-Sepharose CL-6B and Mono Q. column separation of FPLC. From the results of the characteristic studies, these cellulases can be identified as enzyme C_x. |
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| Keywords: | cellulase earthworm cellulase C_1 cellulase C_x enzyme purification |
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