| 响应面法对出芽短梗霉黑色素提取工艺的研究 |
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| 引用本文： | 汪建明,赵博,乔长晟.响应面法对出芽短梗霉黑色素提取工艺的研究[J].井冈山大学学报（自然科学版）,2013(4):19-27. |
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| 作者姓名： | 汪建明 赵博 乔长晟 |
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| 作者单位： | [1]食品营养与安全教育部重点实验室,天津科技大学食品工程与生物技术学院,天津300457 [2]天津北洋百川生物技术有限公司,天津300457 [3]天津市工业微生物重点实验室,工业发酵微生物教育部重点实验室,天津科技大学生物工程学院,天津300457 |
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| 基金项目： | 天津市2011年科技支撑计划重点基金项目（11ZCKFC01800）;天津市东丽区2010年科委项目“传统调味品非热力抑菌技术的集成与示范” |
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| 摘 要： | 本研究以出芽短梗霉发酵液为原料提取黑色素,在单因素实验基础上选取实验因素与水平,用二水平实验确定影响黑色素色价的主要因素,然后根据Box-Behnken中心组合实验设计原理采用三因素三水平的响应面分析法,得到最佳提取工艺为:酸沉时间90 min、乙醇添加量93.17%、乙醇浓度90.42%。在此条件下提取黑色素色价可达44.8μ/mL。
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| 关 键 词： | 芽短梗霉 黑色素 响应面法 提取 |
EXTRACTION TECHNOLOGY OF THE MELANIN FROM AUREOBASIDIUMPULLULANS USING RESPONSE SURFACE METHOD |
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| WANG Jian-ming,ZHAO Bo,QIAO Chang-sheng.EXTRACTION TECHNOLOGY OF THE MELANIN FROM AUREOBASIDIUMPULLULANS USING RESPONSE SURFACE METHOD[J].Journal of Jinggangshan University(Natural Sciences Edition),2013(4):19-27. |
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| Authors: | WANG Jian-ming ZHAO Bo QIAO Chang-sheng |
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| Institution: | 1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin University of Science ＆ 2. Tianjin Peiyang Biotnms Biological Technology Company, Tianjin 300457, China; 3. Thmjin Key Laboratory of Industry Microbiology, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology, Tianjin 300457, China) |
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| Abstract: | The extraction of melanin from Aureobasidium pullulans fermented liquid was studied in this paper. On the basis of single factor experiments, the main factors that influence the valeur of melanin were determined by two-level experiment, and then the optimum conditions for the extraction of the melanin from A. pullulans fermented liquid were obtained through a three-variable, three-level Box--Benhnken center--united experiment design and response surface methodology. The results showed that the optimum conditions were as follows： acid sinking time 90 rain, ethanol add volume 93.17 % and ethanol concentration90.42 %. Under such conditions, the maximal valeur of melanin was up to 44.8 μ/mL. |
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| Keywords: | Aureobasidium pullulans melanin response surface methodology extraction |
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