一株好氧反硝化细菌的筛选鉴定及其脱氮效果

经过富集培养和反硝化能力测定,从城市污水处理厂活性污泥中筛选得到1株好氧反硝化细菌。通过16S rDNA同源性分析对该菌进行分子鉴定,并将菌接种到模拟富营养化水体中以探究其对系统中硝态氮和总氮的去除能力。结果表明,该菌属于Klebsiella sp.,命名为HLNR05。菌株HLNR05在24 h内NO3--N的去除率达96.6%,TN去除率达92.1%,而且其从培养体系中完全去除的氮明显多于同化到细胞的氮。利用HLNR05处理模拟富营养化废水,结果发现其在24 h内对模拟废水中的NO3--N和TN去除率达91.4%和88.2%。表明菌株HLNR05具有较强的好氧反硝化能力。

ISOLATION,IDENTIFICATION OF AN AEROBIC DENITRIFIER AND ITS EFFECT OF NITROGEN REMOVAL

An aerobic denitrifying bacteria was isolated from the sludge of municipal waste water treatment plant by enrichment and denitrification potential test, and identified through 16 S rDNA sequence analysis. Then it was inoculated into simulation of eutrophication system, aiming to enhance the denitrification capacity. The strain was identified as Klebsiella sp. and designated HLNR05. It could remove 96.6% or 92.1% of the nitrate nitrogen and the total nitrogen at 24 h, respectively. Moreover, the nitrogen which was removed from the inoculation system is higher than assimilated into the cell. In addition, it could remove 91.4% and 88.2% of the nitrate nitrogen and the total nitrogen in the simulated eutrophic wastewater system at 24 h, respectively. It can be concluded that the aerobic denitrifier has a strong ability of aerobic denitrification.