Cloning and characterization of vernalization-related gene (ver203F) cDNA 3′ end |
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| Authors: | Weidong Yong Kang Chong Tiebing Liang Zhihong Xu Kehui Tan Zhiqing Zhu |
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| Institution: | (1) Research Center for Molecular and Developmental Biology, Institute of Botany, Chinese Academy of Sciences, 100093 Beijing, China;(2) Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, 200032 Shanghai, China |
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| Abstract: | Based on the cDNA fragment sequence of vernalization-related geneverc203 cloned by differential screening in our lab, the 5′ primer has been designed. The cDNA 3′ end ofver203 gene (1 197 bp) has been cloned by the RACE method. And it is identified by Northern blotting that its expression is special
in vernalization treatment. After comparing the sequence in the nucleotide sequence databases of Genbank, EMBL and DDBJ, the
gene has homology withHordeum vulgare jesmonate-induced protein gene. It is suggested that this gene might be related to the signal transduction mediated by jamonate. |
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| Keywords: | wheat vernalization-related gene cDNA 3′ end RACE |
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