| 超声辅助磷酸提取油橄榄叶中羟基酪醇工艺研究 |
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| 引用本文： | 李春燕,颜军,刘嵬,苟小军.超声辅助磷酸提取油橄榄叶中羟基酪醇工艺研究[J].成都大学学报(自然科学版),2013,32(4):328-331. |
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| 作者姓名： | 李春燕 颜军 刘嵬 苟小军 |
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| 作者单位： | 成都大学药食同源植物资源开发四川省高校重点实验室,四川成都,610106;成都大学药食同源植物资源开发四川省高校重点实验室,四川成都,610106;成都大学药食同源植物资源开发四川省高校重点实验室,四川成都,610106;成都大学药食同源植物资源开发四川省高校重点实验室,四川成都,610106 |
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| 摘 要： | 建立HPLC测定羟基酪醇的方法，通过单因素实验和正交实验优化，以羟基酪醇的含量为考察指标，确定超声辅助磷酸提取羟基酪醇的最佳工艺，研究超声辅助磷酸提取油橄榄叶中羟基酪醇的工艺．实验结果显示：羟基酪醇在3．75—120μg／mL范围内线性关系良好；超声辅助磷酸提取羟基酪醇的最佳奈件为，磷酸浓度为o,2mol／L，液料比为40：l（mL：g），超声时间为60rain，此条件下羟基酪醇的含量为5．13mg/g．该方法工艺简单，处理量大，可为油橄榄叶中羟基酪醇的提取提供依据．
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| 关 键 词： | 油橄榄叶 羟基酪醇 磷酸 高效液相色谱法 |
Study on Ultrasound-assisted Phosphoric Acid Extraction Technology of Hydroxytyrosol from Olive Leaves |
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| LI Chunyan,YAN Jun,LIU Wei,GOU Xiaojun.Study on Ultrasound-assisted Phosphoric Acid Extraction Technology of Hydroxytyrosol from Olive Leaves[J].Journal of Chengdu University (Natural Science),2013,32(4):328-331. |
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| Authors: | LI Chunyan YAN Jun LIU Wei GOU Xiaojun |
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| Institution: | (The Key Laboratory of Medicinal and Edible Plants Resources Exploitation of Higher Education Institutes of Sichuan Province, Chengdu University, Chengtu 610106, China) |
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| Abstract: | Optimized by single factor test and orthogonal test, taking the content of hydroxytyrosol as evalu- ation index, the experiment was established to determine hydroxytyrosol by HPLC. The experimental results showed that hydroxytyrosol had a good linear relationship with peak area in the range 3.75 ~ 120 btg/mL. When the optimized ultrasonic extraction conditions of hydmxytyrosol were 2 mol/L phosphoric acid, 40：1 liquid-solid ratio （mL： g） and extraction for 60 rain at normal temperature, the hydroxytymsol content was 5.13 mg/g. We conclude that the method is simple, and has large capacity, and can provide the basis for extraction of hydroxytyrosol from olive leaves. |
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| Keywords: | olive leaves hydroxytyrosol phosphoric acid HPLC |
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