| 藻蓝蛋白裂合酶突变体的构建及其对催化功能的影响 |
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| 引用本文： | 张玲,武栋,叶斌,周明,赵开弘.藻蓝蛋白裂合酶突变体的构建及其对催化功能的影响[J].华中师范大学学报(自然科学版),2006,40(2):256-259. |
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| 作者姓名： | 张玲 武栋 叶斌 周明 赵开弘 |
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| 作者单位： | 1. 华中科技大学,环境科学与工程学院,武汉,430074
2. 武汉生物工程学院,生物技术系,武汉,430415 |
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| 摘 要： | 在序列分析的基础上，使用PCR技术构建了藻蓝蛋白裂合酶CpcE／CpcF基因的缺失突变体：cpcE（42～276）、cpcE（1～274）、cpcE（1～272）、cpcF（1～160）、cpcF（10～213）．突变体基因克隆至表达载体pET 30a（＋）后，利用体外重组实验对表达蛋白的酶活性进行了研究．揭示了缺失区域在酶催化过程中的作用．
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| 关 键 词： | 藻蓝蛋白 裂合酶 缺失突变体 体外重组 |
| 文章编号： | 1000-1190（2006）02-0256-04 |
| 收稿时间： | 2005-09-10 |
| 修稿时间： | 2005-09-10 |
Construction of deletion mutants and its effects on the catalytic function of phycocyanin lyase in Mastigocladus laminosus |
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| ZHANG Ling,WU Dong,YE Bin,ZHOU Ming,ZHAO Kai-hong.Construction of deletion mutants and its effects on the catalytic function of phycocyanin lyase in Mastigocladus laminosus[J].Journal of Central China Normal University(Natural Sciences),2006,40(2):256-259. |
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| Authors: | ZHANG Ling WU Dong YE Bin ZHOU Ming ZHAO Kai-hong |
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| Institution: | 1. Huazhong University of Science and Technology, College of Environmental Science and Engineering, Wuhan 430074; 2. Department of Bioteehnology, Wuhan Bioengineering Instiute, Wuhan 430415 |
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| Abstract: | On the basis of sequence analysis,deletion mutants of phycocyanin lyase CpcE/CpcF in Mastigocladus laminosus were constructed: cpcE(42-276),cpcE(1-274),cpcE(1-272),cpcF(1-160),cpcF(10-213).Gene of the deletion mutants was then cloned into expression vector pET 30a(+).The activities of the expressed proteins were studied through the in vitro reconstitution test,which showed the function of deleted domain in the enzyme catalysis. |
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| Keywords: | Mastigoeladus laminosus phyeoeyanin CpeE/F deletion mutation |
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