| 野生及人工培养香菇和黑木耳中粗多糖含量测定 |
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| 引用本文： | 周玲,唐静,李娇,丁洁,刘波.野生及人工培养香菇和黑木耳中粗多糖含量测定[J].大理学院学报,2008,7(8):58-60. |
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| 作者姓名： | 周玲 唐静 李娇 丁洁 刘波 |
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| 作者单位： | 大理学院药学院,云南,大理,671000 |
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| 基金项目： | 大理学院校科研和教改项目 |
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| 摘 要： | 目的：测定野生及人工培养的香菇、黑木耳中多糖含量。方法：采用苯酚-硫酸法测定其多糖含量。结果：在10～60μg／ml线性范围内，相关系数r=0．9994，平均回收率为99．45％，RSD为0．47％（n=6）。野生香菇和黑木耳中多糖含量分别为9．52％和8．54％，人工培养的香菇和黑木耳中多糖含量分别为8．36％和7．77％。结论：此方法操作方便，结果准确，为香菇和黑木耳的进一步研究提供了可靠的依据。
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| 关 键 词： | 多糖 苯酚-硫酸法 香菇 黑木耳 |
Determination of Content of Polysaccharides in Wild and Man-cultivated Lentinus edodes and Auricularia auricular |
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| ZHOU Ling,Tang Jing,LI Jiao,DING Jie,LIU bo.Determination of Content of Polysaccharides in Wild and Man-cultivated Lentinus edodes and Auricularia auricular[J].Journal of Dali University,2008,7(8):58-60. |
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| Authors: | ZHOU Ling Tang Jing LI Jiao DING Jie LIU bo |
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| Institution: | (College of Pharmacy, Dali University, Dali Yunnan, 671000, China) |
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| Abstract: | Objective： To determine the contents of polysaccharides in wild and man-cultivated Lentinus edodes and Auricularia auricular, and compare the diversity of the contents respectively. Methods： Phenol-sulfuric acid method was used to determine the contents of amylase. Results： Within the range of 10-60ug/ml, the regression equation was A=0.0149C-0.0579, r=0.9994. The average recovery was 99.45% with RSD of 0.47% （n=6）. The contents were 9.52% and 8.54% for wild Lentinus edodes and Auricularia auricular and 8.36% and 7.77% for man-cuhivated ones respectively. Conclusion： The method was simple, accurate and easy to perform. |
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| Keywords: | polysaccharides phenol-sulfuric acid method Lentinus edodes Auricularia auricular |
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