长足大竹象产卵行为研究

【目的】探索长足大竹象(Cyrtotrachelus buqueti Guerin-Meneville)的产卵行为规律。【方法】通过室内饲养观察和视频捕捉相结合的方法,记录了长足大竹象完整的产卵过程,比较了寄主种类和生理状态对其产卵行为的影响。【结果】长足大竹象完整的产卵过程包括爬行、停留、探测、刻槽、产卵器伸出、产卵和隐藏(修饰)共7个阶段。室内试验表明,长足大竹象完整产卵时间为(69.85±1.64)min,其中爬行(25.56±0.79)s,停留(45.57±0.70)s,探测(1.19±0.03)min,刻槽(38.51±0.42)min,产卵器伸出(8.89±0.19)s,产卵(2.65±0.05)min,修饰(23.87±0.74)min。雌虫初次产卵时,在爬行、停留、探测、刻槽、产卵时间上显著高于有产卵经历的雌虫(P0.05);除产卵器伸出和产卵的频次差异不显著外,雌虫初次产卵中其他行为的频次都显著长于再次产卵(P0.05)。雌虫在健康竹笋上刻槽、产卵和修饰历期显著长于在受损竹笋(P0.05),而在健康竹笋上各行为的频次均高于受损竹笋的相应阶段(P0.05)。长足大竹象在不同种类竹笋上的产卵时间存在差异,在慈竹上,雌虫的爬行、停留、探测历期明显短于其余4种竹类(P0.05),而刻槽历期最长((39.37±0.54)min)(P0.05)。室内观察发现长足大竹象在第3至9天之间达到产卵高峰期。【结论】长足大竹象产卵行为受产卵经历的影响,在不同寄主上的产卵行为差异显著。

Oviposition behavior of Cyrtotrachelus buqueti Guerin-Meneville

Objective] This study aimed to explore the patterns of oviposition behavior of Cyrtotrachelus buqueti Guerin-Meneville. Method] We examined the complete oviposition process through laboratory rearing and video recording, and then compared the effects of different species and physiological status of hosts on oviposition behavior. Result] The re-sults showed that the complete oviposition process of C. buqueti included creeping, inactivity, probing, gnawing nidus, ovipositor protrusion, oviposition, and hiding. In the laboratory, the complete oviposition process was observed to last for (69. 85± 1. 64) min. The duration of the component behaviors of the oviposition process were as follows: creeping (25. 56± 0. 79) s; inactivity ( 45. 57 ± 0. 70) s; probing ( 1. 19 ± 0. 03) min; gnawing nidus ( 38. 51 ± 0. 42) min;ovipositor protrusion (8. 89±0. 19)s; oviposition (2. 65±0. 05)min; and hiding (23. 87±0. 74)min. For females ovi-positing for the first time, the times spent creeping, inactivity, probing, gnawing nidus, and ovipositing were significant-ly longer than for those females that had oviposited previously (P<0. 05). With the exception of the frequencies of ovi-positor protrusion and oviposition, the frequencies of the other oviposition behaviors of inexperienced females were con-siderably higher compared to those of experienced females (P<0. 05). C. buqueti females preferentially selected healthy hosts for oviposition. When females oviposited on healthy bamboo shoots, gnawing nidus, oviposition, and hiding times were considerably longer (P<0. 05), and the frequency of all these behaviors was significantly higher (P<0. 05) com-pared with females ovipositing on damaged bamboo shoots. The oviposition time of C. buqueti varied with different hosts. When ovipositing on Neosinocalamus affinis, the creeping, inactivity, and probing times of females were significantly shorter compared with those on the other four examined hosts (P<0. 05), and the time for gnawing nidus was the longest ((39. 37±0. 54) min) (P<0. 05). The peak of C. buqueti oviposition in the laboratory occurred between the third days to ninth days. Conclusion] The oviposition behavior of C. buqueti was influenced by oviposition experience, and differed significantly on different hosts.