| 一种适合杨树叶片的蛋白质提取方法 |
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| 引用本文： | 袁坤,王明庥,黄敏仁.一种适合杨树叶片的蛋白质提取方法[J].南京林业大学学报(自然科学版),2007,31(3):119-121. |
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| 作者姓名： | 袁坤 王明庥 黄敏仁 |
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| 作者单位： | 国家林业局、江苏省林木遗传和基因工程重点实验室(南京林业大学),江苏,南京,210037 |
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| 摘 要： | 高质量的蛋白样品制备是进行双向电泳的前提条件.杨树叶片中因含有丰富的多酚、色素、多糖等物质,给蛋白质的提取带来了困难.为了找到一种适合杨树叶片的蛋白质提取方法,以南林895杨为实验材料,采用三氯乙酸-丙酮沉淀法对杨树叶片蛋白质进行提取.对所提取的蛋白分别用考马斯亮蓝染色法和银染法进行检测,得到了良好的双向电泳图谱.
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| 关 键 词： | 杨树叶片 蛋白质提取 双向电泳 杨树叶 蛋白质 提取 方法 Leaves Poplar Protein Extraction 电泳图谱 检测 银染法 染色法 考马斯亮蓝 丙酮沉淀法 三氯乙酸 实验材料 南林 物质 多糖 色素 多酚 |
| 文章编号： | 1000-2006（2007）03-0119-03 |
| 修稿时间： | 2006-11-062007-03-01 |
A Method Suitable for Protein Extraction of Poplar Leaves |
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| YUAN Kun,WANG Ming-xiu,HUANG Min-ren.A Method Suitable for Protein Extraction of Poplar Leaves[J].Journal of Nanjing Forestry University(Natural Sciences ),2007,31(3):119-121. |
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| Authors: | YUAN Kun WANG Ming-xiu HUANG Min-ren |
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| Institution: | The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province(Nanjing Forestry University |
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| Abstract: | High-quality protein sample preparation is the premise of two-dimensional gel electrophoresis(2-DE).Poplar leaves contain abundant interfering compounds such as polyphenols,pigments,polysaccharides and so on,which make it difficult to extract proteins.In order to find a method suitable for protein extraction of poplar leaves,the poplar of NL895 was used as the experimental material.The method of trichloroacetic acid(TCA)-acetone was adopted to extract proteins.Coomassie brilliant blue(CBB) and silver staining were used respectively to detect the extracted proteins.Good protein patterns were obtained.This paper provides a basis for poplar proteomics research in our lab. |
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| Keywords: | Poplar leaves Protein extraction Two-dimensional gel electrophoresis |
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