| 日本鳗鲡肝肾病原菌及其防治中药的研究 |
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| 引用本文： | 韩金钢,关瑞章,黄文树,靳恒,郭松林.日本鳗鲡肝肾病原菌及其防治中药的研究[J].集美大学学报(自然科学版),2011,16(5):327-334. |
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| 作者姓名： | 韩金钢 关瑞章 黄文树 靳恒 郭松林 |
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| 作者单位： | 集美大学水产学院,福建厦门,361021 |
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| 基金项目： | 农业部公益性行业(农业)科研专项，福建省科技计划重点项目，福建省教育厅重点资助项目，厦门市科技计划重点项目 |
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| 摘 要： | 从发病日本鳗鲡（Anguilla japonica）的肝脏和肾脏分离到优势菌并命名为AJL01,经人工感染试验证实该菌为引起日本鳗鲡肝肾病的病原菌,其半数致死量为2.6×103 cfu/g;通过菌体、菌落形态特征、GYZ-15EV鉴定系统和16S rDNA同源性等综合鉴定分析,确认AJL01为迟钝爱德华氏菌（Edwar...
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| 关 键 词： | 日本鳗鲡 爱德华氏菌 16S rDNA 中草药 |
Investigations into the Pathogenic Bacteria from Liver and Kidney of Anguilla japonica and the Chinese Herbals for Controlling |
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| HAN Jin-gang,,GUAN Rui-zhang,HUANG Wen-shu,JIN Heng,GUO Song-lin,Fujian Province,.Engineering Research Center of the Modern Industrial Technology for Eel,Ministry of Education,China.Investigations into the Pathogenic Bacteria from Liver and Kidney of Anguilla japonica and the Chinese Herbals for Controlling[J].the Editorial Board of Jimei University(Natural Science),2011,16(5):327-334. |
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| Authors: | HAN Jin-gang GUAN Rui-zhang HUANG Wen-shu JIN Heng GUO Song-lin Fujian Province Engineering Research Center of the Modern Industrial Technology for Eel Ministry of Education China |
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| Institution: | HAN Jin-gang1,2,3,GUAN Rui-zhang1,HUANG Wen-shu1,JIN Heng1,GUO Song-lin1,3(1.Fisheries Colleges,Jimei University,Xiamen 361021,China,2.Key Laboratory of Science and Technology for Aquaculture and Food Safety(Jimei University),Fujian Province,3.Engineering Research Center of the Modern Industrial Technology for Eel,Ministry of Education,China) |
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| Abstract: | A dominant strain of bacteria which was isolated from the liver and kidney of the diseased Anguilla japonica was named as AJL01.Artificial injection test proved that bacteriaum AJL01 was the pathogenic bacteriaum that triggered this ulcer disease.It was also showed that E.tarda was virulent against eel with a LD50 value of 2.6×103cfu/g.According to morphogenetic features,physiological and biological characteristics and 16S rDNA homology comparison of the bacteriaum,the pathogenic bacteriaum was Edwardsiella tarda;Moreover,the minimum inhibitory concentration（MIC）and minimum bactericidal concentration（MBC）of five Chinese herbals and their combination compounds between each other against Edwardsiella tarda were measured.The results showed that five Chinese herbals could kill Edwardsiella tarda,the range of MIC and MBC were from 0.5 mg/mL to 3.0 mg/mL.Six of the ten compounds could synergistically inhibit the growth of the bacterium,especially the Rheum Palmatum and Puncia Granatum,Puncia Granatum and Rhizoma Polygoni,Scutellaria Baicalensis and Rheum Palmatum.The FIC was less than 0.5,and the MIC and MBC less than or equal to 0.47mg/mL. |
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| Keywords: | Anguilla japonica Edwardsiella tarda 16S rDNA Chinese medicinal herb |
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