| 产Nisin突变株发酵条件的优化 |
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| 引用本文： | 张贺迎,武金霞,赵洁.产Nisin突变株发酵条件的优化[J].河北大学学报(自然科学版),2008,28(3):291-294. |
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| 作者姓名： | 张贺迎 武金霞 赵洁 |
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| 作者单位： | 1.河北大学,生物工程技术研究中心,河北,保定 071002； 2.河北大学,生命科学学院,河北,保定 071002 |
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| 基金项目： | 河北省生物工程重点学科项目
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河北省生物学强势特色学科资助项目 |
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| 摘 要： | 研究了产Nisin突变株WY-4的发酵条件,最适接种量为5%,最适发酵温度为30℃,最适起始pH值为6.75,采用流加1 mol/L的NaOH溶液来控制发酵过程中发酵液pH值不低于6,在此条件下,Nisin的效价达到718.98 IU/mL,比对照提高了29.14%.
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| 关 键 词： | 乳链球菌素 防腐剂 发酵 |
| 文章编号： | 1000-1565(2008)03-0291-04 |
| 修稿时间： | 2007年12月15 |
Optimization of the Fermentation Conditions of Mutant WY-4 Which Produces Nisin |
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| ZHANG He-ying,WU Jin-xia,ZHAO Jie.Optimization of the Fermentation Conditions of Mutant WY-4 Which Produces Nisin[J].Journal of Hebei University (Natural Science Edition),2008,28(3):291-294. |
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| Authors: | ZHANG He-ying WU Jin-xia ZHAO Jie |
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| Abstract: | The fermentation conditions of the mutant WY-4 which produces Nisin were optimized as follows: inoculating quantity(5%),temperature(30 ℃),and the original pH(6.75),pH of the ferment liquid being controlled at pH=6 by adding 1 mol/L NaOH continuously during the fermentation.Under these conditions,Nisin activity increased remarkably,and its concentration reached 718.98 IU/mL,which was 29.14% higher than that of control. |
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| Keywords: | Nisin preservative fermentation |
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