| 红豆杉细胞在反应器中培养动力学的研究 |
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| 引用本文： | 余斐,吴诚德,罗杰,刘凌.红豆杉细胞在反应器中培养动力学的研究[J].华中科技大学学报(自然科学版),2001,29(Z1):52-54. |
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| 作者姓名： | 余斐 吴诚德 罗杰 刘凌 |
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| 作者单位： | 华中科技大学生命科学与技术学院 |
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| 基金项目： | 国家高技术研究发展计划 (102-12-06-01);国家"九五”重点科技攻关项目(96-C02-03-01). |
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| 摘 要： | 应用气升式生物反应器及搅拌式生物反应器悬浮培养红豆杉细胞,得到细胞生长和紫杉醇合成动力学曲线.经过反应器动力学的比较,结果表明红豆杉细胞较适于在机械搅拌式反应器中培养.
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| 关 键 词： | 红豆杉细胞 紫杉醇 生物反应器 气升式 搅拌式 |
| 文章编号： | 1000-8616(2001)1-0052-03 |
| 修稿时间： | 2000年7月10日 |
The Comparative Study on Taxus chinensis Cell Suspension Culture in Airlift Bioreator and Stirred Tank Bioreactor |
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| Yu Fei Wu Chengde Luo Jie Liu Ling College of Life Sci. & Tech.,HUST,Wuhan ,China..The Comparative Study on Taxus chinensis Cell Suspension Culture in Airlift Bioreator and Stirred Tank Bioreactor[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2001,29(Z1):52-54. |
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| Authors: | Yu Fei Wu Chengde Luo Jie Liu Ling College of Life Sci & Tech HUST Wuhan China |
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| Institution: | Yu Fei Wu Chengde Luo Jie Liu Ling College of Life Sci. & Tech.,HUST,Wuhan 430074,China. |
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| Abstract: | The growth of plant cell cultures of Taxus chinensis in the airlift bioreactor and stirred tank bioreactor is studied. On the basis of the knowledge the secondary metabolism in cultured Taxus chinensis cells, the comparison research is done. The culture growth and taxol production are all analyzed. The experimental results show that culturing in the stirred tank bioreactor is more suitable for Taxus chinensis cells. The maximum dry cells weigh 18.5?g/L and the maximum taxol concentration of 20.2?mg/L are obtained after 30 days. |
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| Keywords: | Taxus chinensis cell taxol bioreactor airlift stirred |
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