| 硒对NO诱导的内皮细胞损伤的抑制机制研究 |
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| 引用本文： | 邓英,黄开勋,等.硒对NO诱导的内皮细胞损伤的抑制机制研究[J].华中科技大学学报(自然科学版),2002,30(5):101-104. |
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| 作者姓名： | 邓英 黄开勋 |
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| 作者单位： | 华中科技大学药物研究所
(邓英，黄开勋)，华中科技大学药物研究所(徐辉碧) |
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| 基金项目： | 国家自然科学基金资助项目 (2 99710 12 ) |
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| 摘 要： | 用外源性NO供体S－亚硝基谷胱甘肽（GSNO）处理人脐静脉内皮细胞系ECV－304细胞，研究其对细胞的损伤机制，并探讨硒在这一过程中的保护作用，通过MTT法测定细胞存活率，分光光度法测定细胞LDH漏出率及细胞脂质过氧化水平，采用荧光标记技术研究细胞膜流动性变化，结果表明，NO可引起细胞脂质过氧化水平升高，细胞膜流动性下降，导致ECV－304细胞损伤，其作用具有浓度效应，细胞内硒可通过抑制细胞脂质过氧化水平及细胞膜流动性变化从而抑制NO诱导的细胞损伤。
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| 关 键 词： | S－亚硝基谷胱甘肽 内皮细胞损伤 脂质过氧化 细胞膜流动性 NO 硒 |
Study of the inhibition of selenium on NO-induced cell injury in human umbilical vein endothelial cells and its mechanism |
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| Deng Ying Huang Kaixun Xu Huibi Medicant Institute. of Chemistry, Huazhong Univ. of Sci. and Tech.,Wuhan ,China..Study of the inhibition of selenium on NO-induced cell injury in human umbilical vein endothelial cells and its mechanism[J].JOURNAL OF HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY.NATURE SCIENCE,2002,30(5):101-104. |
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| Authors: | Deng Ying Huang Kaixun Xu Huibi Medicant Institute of Chemistry Huazhong Univ of Sci and Tech Wuhan China |
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| Institution: | Deng Ying Huang Kaixun Xu Huibi Medicant Institute. of Chemistry, Huazhong Univ. of Sci. and Tech.,Wuhan 430074,China. |
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| Abstract: | The damage of S nitrosoglutathione(GSNO), a donor of NO, on human umbilical vein endothelial cells and the protective effect and its mechanism of cellular selenium in this process were studied. Cell viability, the change of membrane fluidity, LDH activity in bath and lipid peroxidation injury were investigated by MTT assay, fluorometric technique and spectrophotometric method, respectively. The results showed that NO induced a concentration dependant lipid peroxidation injury and decreasing of membrane fluidity, resulted in cell damage characterized by the increasing of LDH activity in medium and decreasing of cell activity. Cellular selenium could protect cells from NO induced injury by inhibiting lipid peroxidation, changing membrane fluidity. |
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| Keywords: | S nitrosoglutathione endothelial cell lipid peroxidation membrane fluidity NO selenium |
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