| Electroporation： A New Approach Enhancing Antitumor Effects of Cytoxan |
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| 引用本文： | 杨孔,YUE Bisong,Wang Zishu,ZOU Fangdong,Zhao Ermi,WANG Baoyi,Zhang Hong.Electroporation： A New Approach Enhancing Antitumor Effects of Cytoxan[J].高技术通讯(英文版),2003,9(3):36-41. |
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| 作者姓名： | 杨孔 YUE Bisong Wang Zishu ZOU Fangdong Zhao Ermi WANG Baoyi Zhang Hong |
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| 作者单位： | [1]CollegeofLifeSdenees,SichuanUniversity,Chengdu610064,P.R.China [2]RadioDepartmentofSichuanUniversity,Chengdu610064,P.R.China) |
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| 基金项目： | theNationalNaturalScienceFundationofChina (No:60 0 710 2 4)andScienceandTechnologyFundofSichuanProvince(0 2SY0 2 9 12 7) |
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| 摘 要： | Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (EPs) inducing cell membrane pored (eleetroporation) are used as a means of delivering antitumor drugs to the cytoplasm of cancer cells. In vitro, with scan electromicroscope (SEM) and Trypan blue staining examination, the best parameter of EPs of electroporation is studied by the S-180 cells exposed to EP with various voltages, pulses, capacitance. The best parameter of EP of electroporation is 600V/cm, 6 pulses, 10 μF. In the in vivo study, ECT is studied with the Cytoxan (CTX) injected directly into the tumor followed immediately by a local EP at the tumor site.Four parameters, which include the tumor inhibitory ratio, the curing ratio and the vas capillareof tumor, the tumor‘s histopathological characteristics are determined and compared among the ECT group, the control group, the EP-only group and the drug-only group. The results indicate that the antitumor effect of CTX is sitrnifiezntlt enhanced by electroporation.
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| 关 键 词： | 癌得星 癌症治疗 抗癌作用 电穿孔 电化学治疗 |
Electroporation: A New Approach Enhancing Antitumor Effects of Cytoxan |
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| YUE Bisong,Wang Zishu,ZOU Fangdong,Zhao Ermi,WANG Baoyi,Zhang Hong.Electroporation: A New Approach Enhancing Antitumor Effects of Cytoxan[J].High Technology Letters,2003,9(3):36-41. |
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| Authors: | YUE Bisong Wang Zishu ZOU Fangdong Zhao Ermi WANG Baoyi Zhang Hong |
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| Abstract: | Electrochemotherapy (ECT) is a novel cancer treatment in which electric pulses (Eps) inducing cell membrane pored (electroporation) are used as a means of delivering antitumor drugs to the cytoplasm of cancer cells. In vitro, with scan electromicroscope (SEM) and Trypan blue staining examination, the best parameter of Eps of electroporation is studied by the S-180 cells exposed to EP with various voltages, pulses , capacitance. The best parameter of EP of electroporation is 600V/cm, 6 pulses, 10 μF. In the in vivo study, ECT is studied with the Cytoxan (CTX) injected directly into the tumor followed immediately by a local EP at the tumor site. Four parameters, which include the tumor inhibitory ratio, the curing ratio and the vas capillare of tumor, the tumor's histopathological characteristics are determined and compared among the ECT group, the control group, the EP-only group and the drug-only group. The results indicate that the antitumor effect of CTX is significantly enhanced by electroporation. |
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| Keywords: | electrochemotherapy electroporation Cytoxan S-180 tumor effect |
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