九龙棘蛙和棘胸蛙的染色体比较研究

本文报道了福建蛙类新纪录——九龙棘蛙

Chromosme Studies of Rana jiulongensis and R.spinosa in Mount Wuyi of Fujian

The karyotype and Ag-NOR, of Rana jiulongensis in Mount wuyi of Fujian were studied using preparations of bone marrow cells, and also, comparisons of the karyotypes of four species in the group of "Spine-frogs" (Rana)were made. The results show that the diploid number of R. jiulongensis is 2n=26, consisting of 5 large and 8 small homologous chromosome pairs in accord with the karyotypic characters of the group of "Spine-frogs". The karyotypic characters of R. jiulongensis itself are found to possess 5 pairs (Nos. 2, 3, 4, 9, 13)of submetacentric chromosomes. A pronounced secondary constriction is observed at the short arm of chromosome No. 6. Sometimes, chromosome No. 4 has also a secondary constriction at the short arm. The nucleolar organizer regions(NORS) appear at the position of the secondary constriction of chromosome No. 6 by Ag-staining.Based on comparisons of their karyotypes and morphology, ecology(environment, vocalization etc.), R. jiulongensis and R. spinosa David should be two different species.