| 分光光度法测定微量铁实验方法的改进 |
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| 引用本文： | 朱庆珍,夏红.分光光度法测定微量铁实验方法的改进[J].实验科学与技术,2009,7(6):25-27. |
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| 作者姓名： | 朱庆珍 夏红 |
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| 作者单位： | 1. 苏州农业职业技术学院,实训中心,江苏,苏州,215008
2. 苏州农业职业技术学院,院长室,江苏,苏州,215008 |
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| 摘 要： | 建立了邻二氮菲分光光度法测定微量铁的改进方法，显色剂邻二氮菲的浓度为1．0g／L，缓冲液为NaAc—HAc溶液（pH为4．6），盐酸羟胺溶液浓度为10g／L，用1cm比色皿在510nm的波长处测定显色液的吸光度。Fe^2＋的浓度在0—2μe,／mL的范围内与吸光度成良好的线性关系（为0．9998），测定结果准确度高。
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| 关 键 词： | 邻二氮菲仪 分光光度法 吸光度 |
Improvement Method of Determination of Trace Fe2+ by Spectrophotometry |
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| ZHU Qing-zhen,XIA Hong.Improvement Method of Determination of Trace Fe2+ by Spectrophotometry[J].Experiment Science & Technology,2009,7(6):25-27. |
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| Authors: | ZHU Qing-zhen XIA Hong |
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| Institution: | (a. Training Center; b. Vice-President Office, Suzhou Polytechnic Institute of Agriculture, Suzhou 215008, China) |
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| Abstract: | A impovement method for the determination of trace Fe^2＋ is established. Phenanthroline is 1.0 g/L, the buffer is Sodium acetate-acetate（ pH =4. 6）, Hydroxylamine hydrochloride is 10 g/L, Detected the absorbance at 510 nm. The calibration curve is linear（ r = 0. 999 8 ） and it had a high accuracy. |
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| Keywords: | phenanthroline spectrophotometry absorbance |
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