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九孔鲍肠道及水体中产几丁质酶菌群的测定
引用本文:王志,蔡俊鹏,温其标.九孔鲍肠道及水体中产几丁质酶菌群的测定[J].华南理工大学学报(自然科学版),2005,33(3):87-90.
作者姓名:王志  蔡俊鹏  温其标
作者单位:华南理工大学,轻工与食品学院,广东,广州,510640
基金项目:广东省自然科学基金重点资助项目 (020964),华南理工大学高水平大学建设苗子项目(321 D76020)
摘    要:通过特定的TCBS(Thiosufate-Citrate-Bile Salts-Sucrose)和筛选培养基,对九孔鲍肠道及其养殖水体中菌群的产几丁质酶能力进行了研究.结果表明,在鲍鱼肠道及其养殖水体中均存在有能分泌几丁质酶的微生物.在鲍鱼肠道中,共分离到12株菌株,占总细菌的34.3%,它们分别属于Vibrio fluvialis和Vibrio minicus;在鲍鱼养殖水体中则分离到4株菌株,占总细菌的11.8%,属于Vibrio fluvialis.比较鲍鱼肠道和养殖水体中产几丁质酶菌株的比例及其能力的大小,可以发现前者的比例高于后者,由此表明了鲍鱼肠道对菌株的选择性.

关 键 词:九孔鲍  肠道  养殖水体  细菌  几丁质酶
文章编号:1000-565X(2005)03-0087-04
修稿时间:2004年3月23日

Mensuration of Chitinase-Producing Abilities of Strains Isolated From Digestion Guts and Farming Water of Haliotis Diversicolor Supertexta
Wang Zhi,Cai Jun-peng,Wen Qi-biao.Mensuration of Chitinase-Producing Abilities of Strains Isolated From Digestion Guts and Farming Water of Haliotis Diversicolor Supertexta[J].Journal of South China University of Technology(Natural Science Edition),2005,33(3):87-90.
Authors:Wang Zhi  Cai Jun-peng  Wen Qi-biao
Abstract:The chitinase-producing abilities of strains isolated from both farming water and digestion guts of Haliotis diversicolor supertexta were studied by a special TCBS (Thiosufate-Citrate-Bile Salts-Sucrose) and by screening the culture media. The results indicate that there are chitinase-producing strains in farming water and digestion guts of the abalone. 12 bacteria were isolated from abalone digestion guts, which account for 34.3% of the total bacterial populations in the guts. Species identification shows that these chitinase-producing strains belong to Vibrio fluvialis and Vibrio minicus. From the abalone farming water, 4 chitinase-producing strains were isolated, which account for 11.8% of the total bacterial populations. These 4 strains belong to Vibrio fluvialis. Compared with the farming water, it is clear that there are more chitinase-producing strains in abalone digestion guts, and abalone digestion guts display selectivity toward the chitinase-producing strains.
Keywords:Haliotis diversicolor supertexta  digestion guts  farming water  bacteria  chitinase
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