蛋白质组及其在原虫领域中的研究进展

近十几年来,蛋白质组学的研究技术得到了很大发展。质谱技术,比较蛋白质组方法,研究蛋白质相互作用的酵母双杂交技术等的发展,极大地推进了蛋白质组研究的进程。原虫作为单细胞的真核动物,虽体积微小但能独立完成生命活动的全部生理机能,其中近万种原虫为寄生性原虫,它们常寄生在动物体内或体表使其致病,从而对人类及牲畜造成极大的危害。因此,原虫蛋白质组作为蛋白质组学研究的一个分支也迅速发展起来。到目前为止,世界上已有73种原虫的基因组已完成或正在进行测序工作,并且大部分已经进入了蛋白质组研究的工作。本文综述了一些具有代表性的原虫蛋白质组的研究情况,如：溶组织性阿米巴,布氏锥虫,间日疟原虫等,同时阐述了它们的致病机理、诊断及疫苗等方面的蛋白质组研究。

Progress of the Research on Proteome and Proteome in Protozoan Area

Proteome has become the key part of functional genome research in post-genome era.Initially,its significant as- signment is study of whole set proteins coded in the genome with technologies of 2D-electrophoresis,bioinformatics and pro- tein-protein interaction.Recently,Proteomics technologies have been well improved,including Mass Spectrum,comparative proteome method and yeast two-hybrid technology.Protozoan is a unicellular eukaryote,its size is small but can accomplish all the life activities by itself.There are nearly 10,000 species of parasitical protozoan,which cause severe disease to animal or human.The sequence measuring of 73 protozoan genome is completed or in progress in the world,most of which step into proteomics research.Therefore,protozoan proteomics has achieved much advances.In this article,proteome research of rep- resentative protozoan,such as:Entamoeba histolytica.Trypanosoma bruce,Plasmodium vivax.were reviewed,mainly their nosogenesis,diagnosis,vaccines and so on.