栝楼种子萌发期总黄酮含量变化研究

采用超声辅助提取的方法,研究栝楼种子吸胀前期、吸胀期、萌动期、胚根伸长期、萌芽期及幼苗期6个时期的总黄酮含量及变化规律。各萌发阶段的栝楼种子经石油醚脱脂后,以体积分数70%乙醇溶液为溶剂,采用超声辅助提取法提取总黄酮,以芦丁为标准品,采用比色法测定各萌发阶段栝楼种子总黄酮含量。结果表明,栝楼种子中总黄酮含量较低,约为1 mg/g,整个萌发阶段总黄酮含量呈现先下降后上升的趋势,至幼苗期总黄酮含量已达2.7 mg/g。本研究为进一步认识及开发利用栝楼种子提供了参考,为人工调控栝楼种子中总黄酮含量提供了理论依据。

Research on the change of total flavonoids content of Trichosanthes kirilowii seeds during germination period

With the method of ultrasonic assisted extraction, the content and its change rule of total flavonoids in Trichosanthes kirilowii seeds during six stages of prophase imbibition, imbibition, germination, radicle elongation, bud breaking and seedling were studied. After degreased by petroleum ether, total flavonoids of Trichosanthes kirilowii seeds at different germination stage were extracted with 70% ethanol as solvent by ultrasonic assisted extraction, and determined using rutin as the standard by colorimetry method. The results showed that total flavonoids content in the seeds of Trichosanthes kirilowii was low, about 1 mg/g, and it showed tendency to descend firstly and then ascend during the whole germination process, rising to 2.7 mg/g at seeding stage. This study provides references for further understanding and utilization of Trichosanthes kirilowii seeds, and provides theoretical basis for artificial regulation of total flavonoids content in Trichosanthes kirilowii seeds.