| 高效液相色谱法检测蔗渣半纤维素水解物中的单糖 |
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| 引用本文： | 方宏,曾健智,陈海珊,梁小燕,张厚瑞.高效液相色谱法检测蔗渣半纤维素水解物中的单糖[J].广西科学院学报,2000,16(3):124-126. |
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| 作者姓名： | 方宏 曾健智 陈海珊 梁小燕 张厚瑞 |
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| 作者单位： | 广西植物研究所,桂林,541006 |
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| 基金项目： | 广西自然科学基金，9811015， |
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| 摘 要： | 用高效液相色谱法测定甘蔗渣半纤维素水解物中的单糖含量。以Ｈｙｐｅｒｓｉｌ ＮＨ２柱为色谱分析柱，用示差折光检测器检测，流动相是乙腈：水（８０：２０）。在此色谱条件下，甘蔗渣半纤维素水解物分离成：木糖、阿拉伯糖、果糖和葡萄糖。各单糖呈良好的线性。加样回收率的平均值为９７．３％～９８．７％，此方法简便、快速、准确。
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| 关 键 词： | 高效液相色谱法 甘蔗渣 半纤维素水解物 单糖 |
| 收稿时间： | 2000/5/11 0:00:00 |
Determination of Monosaccharide in Bagasse Hemicellulose Hydrolysate by High Performance Liquid Chromatography |
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| Fang Hong,Zeng Jianzhi,Chen Haishan,Liang Xiaoyan and Zhang Hourui.Determination of Monosaccharide in Bagasse Hemicellulose Hydrolysate by High Performance Liquid Chromatography[J].Journal of Guangxi Academy of Sciences,2000,16(3):124-126. |
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| Authors: | Fang Hong Zeng Jianzhi Chen Haishan Liang Xiaoyan and Zhang Hourui |
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| Institution: | Guangxi Institute of Botany, Guilin, 541006,Guangxi Institute of Botany, Guilin, 541006,Guangxi Institute of Botany, Guilin, 541006,Guangxi Institute of Botany, Guilin, 541006 and Guangxi Institute of Botany, Guilin, 541006 |
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| Abstract: | A method for the determination of monosaccharides in hemicellulose hydrolysate from sugar cane bagasse by HPLC(high performance liquid chromatography) was developed.The operating conditions were Hypersil NH2(150mm×4.6mm i.d) column; mobile phase of V(acetonitrile):V(water)=80:20; flow rate of 1.0 ml/min; detected with differential refractometer. Under this condition, the results are satisfactory. The method is simple, quick and accurate. The average recovery was 97.3% to 98.7%. |
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| Keywords: | HPLC(high performance liquid chromatography) bagasse hemicellulose hydrolysate monosaccharide |
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