| HPLC法测定血浆及尿液中阿普唑仑浓度 |
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| 引用本文： | 李嫄,金岩,段旭.HPLC法测定血浆及尿液中阿普唑仑浓度[J].广西科学,2008,15(4):411-413. |
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| 作者姓名： | 李嫄 金岩 段旭 |
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| 作者单位： | 中国医科大学附属盛京医院药剂科,辽宁,沈阳,110004 |
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| 摘 要： | 采用Agilent Eclipse XDB-C18色谱柱(4.6mm×150mm,5μm),以甲醇-水(60¨40)为流动相,流速1.0ml/min,检测波长254nm,建立高效液相法(HPLC)测定血浆及尿液中阿普唑仑浓度。结果表明,HPLC法测定阿普唑仑和内标物的出峰时间是5.1min和8.73min,分离效果较好;阿普唑仑质控3种浓度(4.0μg/ml,8.0μg/ml,16.0μg/ml)日内和日间的RSD,血浆样品均小于6%,尿样均小于7%,血浆回收率为91.15%～93.69%,尿回收率为86.79%～90.11%,血浆及尿样品线性范围为2.0～64.0μg/ml,相关系数为0.9907。血浆和尿样品中阿普唑仑的最低检出限为2.0μg/ml。此条件可以对中毒药物阿普唑仑进行快速检测并定量,方法简便,准确。
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| 关 键 词： | 阿普唑仑 尿液 血浆 高效液相色谱法 |
| 收稿时间： | 2008/1/27 0:00:00 |
Determination of Alprazola in Human Plasma and Urine by HPLC |
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| LI Yuan,JIN Yan and DUAN Xu.Determination of Alprazola in Human Plasma and Urine by HPLC[J].Guangxi Sciences,2008,15(4):411-413. |
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| Authors: | LI Yuan JIN Yan and DUAN Xu |
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| Institution: | Department of Pharmacy, Affiliated Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, China,Department of Pharmacy, Affiliated Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, China and Department of Pharmacy, Affiliated Shengjing Hospital of China Medical University, Shenyang, Liaoning, 110004, China |
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| Abstract: | |
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| Keywords: | Alprazola urine plasma HPLC |
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