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| 对硝基苯酚降解菌Pseudomonas sp．HY1的分离与活性分析 | |
| 引用本文： | 吴萍,张海燕,李梅,颜忠诚,邱星辉.对硝基苯酚降解菌Pseudomonas sp．HY1的分离与活性分析[J].首都师范大学学报(自然科学版),2009,30(3):25-29. |
| 作者姓名： | 吴萍 张海燕 李梅 颜忠诚 邱星辉 |
| 作者单位： | 1. 首都师范大学生命科学学院,北京,100048;中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室,北京,100101 2. 中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室,北京,100101 3. 首都师范大学生命科学学院,北京,100048 |
| 基金项目： | 中国科学院知识创新工程重要方向项目 |
| 摘 要： | 通过驯化富集，从受污染的土壤中分离出一株降解对硝基苯酚（p-nitrophenol，PNP）的细菌．16SrDNA序列分析鉴定该菌为恶臭假单胞菌Pseudomonas sp．HY1．在有氧，pH7和30℃条件下，该菌能利用PNP为碳源和能源生长并将中等浓度（100mg／L）的PNP快速彻底的降解，高浓度（300mg／L）PNP条件下未检测到菌的生长和降解活性．该菌在15～40℃和pH值5～10的条件下具有降解PNP活性，其中碱性条件（pH8～10）和30℃时活性最高． |
| 关 键 词： | 对硝基苯酚 生物降解 假单胞菌 |
Biodegradation of p-Nitrophenol by Pseudomonas sp.HY1 |
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| Wu Ping,Zhang Haiyan,Li Mei,Yan Zhongcheng,Qiu Xinghui.Biodegradation of p-Nitrophenol by Pseudomonas sp.HY1[J].Journal of Capital Normal University(Natural Science Edition),2009,30(3):25-29. | |
| Authors: | Wu Ping Zhang Haiyan Li Mei Yan Zhongcheng Qiu Xinghui |
| Institution: | 1 College of Life Science;Capital Normal University;Beijing 100048;2 State Key Laboratory of Integrated Management of Pest Insects and Rodents;Institute of Zoology;Chinese Academy of Sciences;Beijing 100101 |
| Abstract: | A bacterial strain （HY1）, capable of using p-nitrophenol （PNP） as a sole source of carbon and energy, was isolated from the pesticide-polluted soil. Analysis of the 16s rDNA gene sequence suggested that Y1 had a close relationship to Pseudomonas putida and was designated as Pseudomonas sp. HY1. Degradation studies showed that PNP was degraded quickly under aerobic condition, complete depletion of 50 mg/L and 100 mg/L of PNP was achieved in 10 h and 24 h respectively by HY1 at pH of 7 and temperature of 30 ℃. No growth of HY1 and PNP degradation by HY1 were detected when PNP concentration reached 300 mg/L in the minimal salt media. The greatest growth of HY1 was observed at temperature of 30 ℃ and alkaline pH （pH 8-10）. |
| Keywords: | para-nitrophenol biodegradation Pseudomonas |
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