树脂吸附法分离纯化匙羹藤总皂苷

比较了D101,NKA,AB-8三种树脂对匙羹藤总皂苷的静态吸附性能,D101的吸附效果较好,吸附量达93.90 mg/g;D101树脂对匙羹藤总皂苷的吸附热力学和动力学研究结果表明,Langmuir方程能很好的描述D101树脂的吸附平衡行为,吸附8 h左右达到平衡,在2.5 h以后吸附速率逐渐平稳;确定了D101树脂分离纯化匙羹藤总皂苷的适宜工艺条件,7.73 g/L匙羹藤总皂苷粗提物溶液以1.5 mL/min的流速通过D101树脂柱进行吸附,然后用蒸馏水和体积分数为10%乙醇洗脱杂质,再用体积分数为50%乙醇洗脱,得到的匙羹藤总皂苷纯度为56.10%,得率为83.83%.D101树脂吸附法可用于匙羹藤总皂苷的分离纯化.

Isolation and purification of total saponins in Gymnema sylvestre by adsorption resin

Adsorption characteristics of three kinds of adsorption resins such as D101, NKA and AB-8 for total saponins of Gymnema sylvestre were investigated. D101 resin had the best adsorption capacity for total saponins with 93.90 mg/g. The results of adsorption thermodynamic and dynamic studies of D101 resin for total saponins of Gymnema sylvestre showed that the adsorption balance could be described by Lang- muir type, the time of adsorption balance was eight hours, and adsorption ratio was stable gradually after two and a half hours. The optimal conditions of isolation and purification for the total saponins of Gymnema sylvestre by D101 resin were obtained. The crude total saponins extract solution with the concentration of 7.73 g/L was first subjected to D101 resin adsorption at the flow rate of 1.5 mL/min. Then the inpurities were eluted down by distilled water and 10% ethanol. The adsorbed total saponins were eluted down by 50% ethanol. The purity of the resulting total saponins of Gymrtema sylvestre was 56.10%. The yield of the total saponins was 83.83%. The total saponins of Gymnema sylvestre could be isolated and purified by D101 resin.