| 水稻基因组中U2snRNA基因连锁的分析 |
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| 引用本文： | 冯征宇,王喜萍,孙崇荣,曹凯鸣.水稻基因组中U2snRNA基因连锁的分析[J].复旦学报(自然科学版),2000,39(3):269-272. |
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| 作者姓名： | 冯征宇 王喜萍 孙崇荣 曹凯鸣 |
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| 作者单位： | 生命科学学院,生物化学系 |
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| 基金项目： | 国家科技部水稻基因组研究计划资助项目 (10 1 0 9 0 5 ) |
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| 摘 要： | 根据Ｕ２ｎＲＮＡ基因的保守性和结构特点设计引物，通过ＰＣＲ法扩增Ｕ２ｓｎＲＮＡ连锁基因间区的ＤＮＡ片段并进行顺序分析，证实在水稻中至少存在一组Ｕ２ｓｎＲＮＡ连锁基因。对水稻总ＤＮＡ的ＰＣＲ分析结果表明，水稻中可能还存在其他形式的连锁基因。ＰＣＲ方法可以有效地应用于结构保守基因的连锁分析中。
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| 关 键 词： | U2snRNA基因 基因连锁 PCR法 水稻 基因组 |
| 文章编号： | 0427-7104(2000)03-0269-04 |
Analysis of the Linked U2snRNA Genes in Rice Genome |
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| FENG Zheng-yu,WANG Xi-ping,SUN Chongrong,CAO Kai-ming.Analysis of the Linked U2snRNA Genes in Rice Genome[J].Journal of Fudan University(Natural Science),2000,39(3):269-272. |
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| Authors: | FENG Zheng-yu WANG Xi-ping SUN Chongrong CAO Kai-ming |
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| Institution: | FENG Zheng-yu
,WANG Xi-ping
,SUN Chong-rong
,CAO Kai-ming
(Department of Biochemistry, School of Life Sciences) |
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| Abstract: | A pair of primers, U2d and U2u, were designed based on the conserved region in U2snRNA gene structure. The spacer fragment between U2snRNA linked genes in recombinant DNA from rice was amplified by PCR and seguenced, demonstrating that at least one U2snRNA gene existed in rice genome. The analysis of PCR product from rice total DNA showed that other linked genes were present in rice genome. Therefore, PCR method should be applicable effectively in analysis of linked genes which possessed conserved sequences. |
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| Keywords: | U2snRNA linked gene PCR |
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