固态培养对产紫杉醇真菌小孢拟盘多毛孢次级代谢物生成影响

激活真菌基因组中沉默基因簇,可令其产生新的次生代谢产物,改变培养条件是有效的激活方法.本文研究了固态培养方法对一株产抗癌药物紫杉醇(Taxol)真菌——小孢拟盘多毛孢(Pestalotiopsis microspora)次级代谢产物生成的影响.实验发现固体发酵能够产生2种液体发酵中没有的产物,对其中一种产物进行了高效液相色谱(HPLC)分离纯化和定量分析.该物质的最高产量达到每克培养基质0.354mg,在HPLC谱上的保留时间和紫杉醇接近,值得进一步研究其结构和生物学活性.近年来,固体发酵技术以其独特性和环保优势,再次受到重视.本实验显示固态培养能够诱导该真菌产生2种稳定的次生代谢物,比单纯液体发酵更有优势.本研究还优化了固体发酵基质、发酵时间等条件,为进一步后续研究提供参考.

Effect of solid-state cultural conditions on biosynthesis of secondary metabolites in taxol-producing fungus P.microspora

Paclitaxel is widely used in the treatment of solid tumors,but its production remains short of demand in the global market.Fermentation of entophytic taxol-producing fungi may solve the shortage issue.However,the taxol yield of production in the laboratory remains low.Solid-state fermentation (SSF) for the production of fungal secondary metabolites has drawn much attention in recent years due to a number of advantages over liquid culture techniques.We in this study used SSF to search for taxol-like molecules in taxolproducing fungus Pestalotiopsis microspora.It was found that in SSF the fungus produced two additional compounds that were absent in liquid-state fermentation.One compound was purified by HPLC with a retention time similar to taxol standard.A maximum yield of 0.354 mg per gram of cultivating matrix was achieved.Therefore change of cultivation method can induce further secondary metabolites in fungus.Culture media and time in solid-state fermentation for P.microspora were also tested.