TGF-β1和Caspase-3的表达及妊娠结局在流产模型孕鼠中的研究

目的研究阻断CD86协同刺激分子对自然流产模型孕鼠母胎界面TGF-β1和Caspase-3的表达及妊娠结局的影响.方法实验组于妊娠第4.5d腹腔注射大鼠抗小鼠CD86单抗,对照组注射大鼠同型IgG2b.于妊娠第13.5d计算胚胎吸收率,用免疫组化测定TGF-β1和Caspase-3的表达,并进行图像分析检测免疫组化染色灰度值（A）.结果①实验组的胚胎吸收率显著低于对照组（P〈0.05）②实验组中TGF-β1蛋白的灰度值明显低于对照组（P〈0.05）,实验组中Caspase-3蛋白的灰度值明显高于对照组（P〈0.05）.结论妊娠早期阻断CD86可使母胎界面中的TGF-β1和Caspase-3分别通过各自不同的途径来发挥免疫耐受作用并且使自然流产模型孕鼠的胚胎吸收率降低至正常妊娠水平.

Study of the Expression of TGF-β1 and Caspase-3 Proteins and Outcome of Pregnancy in Murine Abortion-prone Model

Objective To study the effect of blockage of costimulatory signal CD86 at the time of implantation on the expression of TGF-β1 and Caspase-3 proteins at the maternal-fetal interface and pregnant outcome in routine abortion-prone model. Methods Female pregnant CBA/J mice of Experimental group and Control experimental group were received an i.p injection of 100 ？g of antimouse CD86 mAb in 200 ？1 PBS or irrelevant-isotype matched rat IgG2b on day 4.5 of gestation, respectively. As for the normal pregnant group, nothing was received. Pregnant CBA/J mice were killed on day 13.5 of gestation. Then, the embryo resorption rate was calculated, and the expressions of TGF-β1 and Caspase-3 at the placental and decidual units were detected by using immunohistochemical methods and A values of immunohistochemical staining were detected by image analysis. Results ①The embryo resorption rate in experimental group was significantly reduced in comparison with that in control experimental group（P〈0.05）.②A values of TGF-β1 in experimental group was significantly reduced in comparison with that in control experimental group （P〈0.05）. A values of Caspase-3 in experimental group was significantly increased in comparison with that in control experimental group （P〈0.05）. Conclusions Blockage of costimulatory signal CD86 at the early stage of gestation can induce TGF-β1and Caspase-3 to exert immunologic tolerance by their different mechanisms and reduce the embryo resorption rate of the abortion-prone model to that of normal pregnant level.