七星鲈胚胎发育与水温关系的研究试验

2006年12月15日至18日在福建东山岭南育苗场进行七星鲈(Lateolaberx japonicus)胚胎发育与水温关系的研究试验.试验设定水温为16～24℃,每个温度梯度为1℃,每个实验组设2个重复,每组加入受精卵100粒.结果显示,温度对七星鲈胚胎发育速度的影响非常明显,七星鲈胚胎发育进程随着温度的降低而延长,...     

我国治疗性克隆法律制度的完善

随着治疗性克隆技术的不断深入发展，通过法律制度来规制治疗性克隆技术，避免其可能带来的伦理与法律问题，为克隆技术的健康快速发展创造良好的社会环境，成为目前各国面临的重要问题。当前，我国急需完善法律制度对治疗性克隆加以规范，以保证我国克隆技术的健康发展。     

Identification of an S5n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility

Oryza rufipogon exhibits abundant genetic diversity and contains many elite genes. In this work, a total of 441 accessions of O. rufipogon were genetically examined using the functional marker S5n, revealing that 18 accessions carried the S5n allele with a heterozygous genotype (S5nS5i/j). Of these accessions, 14 were from Guangdong Province, 2 from Guangxi Zhuang Autonomous Region, 1 from Hainan Province and 1 from Jiangxi Province. Further sequence analysis spanning both sides of the deletion in S5n allel...     

四川栽培小麦幼胚培养再分化能力变异性的研究

以N6为基本培养基，对四川栽培小麦品种、品系以及农家品种共45个基因型的幼胚离体培养，进行了植株再生能力变异性的研究。结果表明，供试材料间的形态发生能力存在明显变异，表现为器官建成速度明显不同，芽器官和根器官再生率存在极显著差异，分别变异在15．00％－95．45％和0－95．45％之间，由每个幼胚外植体起源的愈伤组织再生出1．0cm以上的芽数及其芽长均存在极显著差异。     

朝鲜鹌鹑耳蜗的组织发生

以朝鲜鹌鹑胚胎和幼体的内耳为材料，用组织学方法对内耳的发育过程进行研究。孵化３６ｈ，听基板已形成；孵化７２ｈ，听泡形成；孵化４ｄ，基乳突形成；孵化６ｄ，毛细胞分化出来；孵化１６ｄ，内耳结构基本发育完成；出壳后，其结构继续发育完善。 发育结果显示：螺旋神经节细胞和毛细胞优先发育，附属结构在其后发育。     

Combinational electroporation and transplantation approach to studying gene functions in avian embryos

Gene transfection is an indispensable approach for studying gene function since it provides important information on gain- and/or loss-of-function. Chick embryos are also extensively employed for studying bio- logical function since they are easily accessible and can be maintained alive after manipulation. The combination of both techniques presents a powerful approach to under- standing how genes regulate embryo development. Fur- thermore, combining these approaches with tissue transplant techniques make even more attractive for elu- cidate gene function. Electroporation, employing parallelly fashioned electrodes, has been widely used in chick embryos. However, experimenters have been frustrated by unsuccessfully transfection in some embryonic tissue of interest because the electrodes were improperly positioned.We presently demonstrated the different patterns of orga- nizing and positioning the electrodes, in combination with tissue transplantation, to efficiently and specifically trans- fect the chick embryonic head, trunk neural tube, heart tube, somites and neural crest cells with the GFP reporter gene.     

东北龙胆的受精作用及其胚和胚乳的发育(Ⅱ)

东北龙胆的胚囊发育属于蓼型。珠孔受精。花粉管可通过破坏一个助细胞进入胚囊,也可在助细胞与胚囊壁之间进入胚囊。花粉管进入胚囊后释放出的两个精子分别与卵细胞及次生核融合,形成合子及初生胚乳核。受精作用属于有丝分裂前配子融合类型。胚乳发育为核型。当胚发育到4—6个细胞时,在胚囊珠孔瑞的游离核之间先形成细胞壁。细胞壁的形成是以自由壁的方式从珠孔端到合点端自外向内进行。胚的发育属于茄型。     

Development and characterization of interspecific hybrids between Oryza sativa and O. latifolia by in situ hybridization

Oryza sativa and O. latifolia belong to the AA and CCDD genomes of Oryza, respectively. In this study, interspecific hybrids of these species were obtained using the embryo rescue technique. Hybrid panicle traits, such as long awns, small grain, exoteric large purple stigma, grain shattering and dispersed panicles, resemble that of the paternal parent, O. latifolia, whereas there is obvious heterosis in such respects as plant height, tillering ability and vegetative vigor. Chromosome pairing and the genomic components of the hybrid were subsequently investigated using genomic in situ hybridization (GISH) and fluorescent in situ hybridization (FISH) analysis. Based on the mitotic metaphase chromosome numbers of the root tips investigated, the hybrid is a triploid with 36 chromosomes. The genomic constitution of the hybrid is ACD. In the meiotic metaphase I of the hybrid pollen mother cell, poor chro- mosome pairing was identified and most of the chromosomes were univalent, which resulted in com- plete male sterility in the hybrid.