| 一株产紫杉醇的红豆杉内生真菌的分离鉴定 |
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| 引用本文： | 张红涛,邓百万,陈文强,等.一株产紫杉醇的红豆杉内生真菌的分离鉴定[J].陕西理工学院学报(自然科学版),2014(2):41-45. |
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| 作者姓名： | 张红涛 邓百万 陈文强 等 |
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| 作者单位： | [1] 陕西理工学院生物科学与工程学院,陕西汉中723000 [2]陕西省食药用菌工程技术研究中心,陕西汉中723000 [3] 勉县第一中学,陕西勉县724200 |
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| 基金项目： | 基金项目：陕西省教育厅科学研究计划项目（11JK0616）;陕西理工学院科研基金资助项目（SLGKY10-12） |
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| 摘 要： | 用组织研磨涂布法对中国红豆杉中产紫杉醇的内生真菌进行分离,得到45株内生真菌;通过形态学和分子生物学手段对一株产紫杉醇的菌株Tax-4进行鉴定,其结果为黑团壳属(Massaria)。表明从中国红豆杉中分离得到了一株产紫杉醇的黑团壳属内生真菌,丰富了产紫杉醇内生真菌的资源。
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| 关 键 词： | 红豆杉 紫杉醇 内生真菌 分离鉴定 |
Isolation and identification of an endophytic taxol-producing fungus associated with Taxus chinensis |
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| ZHANG Hong-tao,DENG Bai-wan,CHEN Wen-qiang,LIU Kai-hui,DING Xiao-wei,GU Dong-sheng,LI Hua.Isolation and identification of an endophytic taxol-producing fungus associated with Taxus chinensis[J].Journal of Shananxi University of Technology:Natural Science Edition,2014(2):41-45. |
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| Authors: | ZHANG Hong-tao DENG Bai-wan CHEN Wen-qiang LIU Kai-hui DING Xiao-wei GU Dong-sheng LI Hua |
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| Institution: | ZHANG Hong-tao, DENG Bai-wan, CHEN Wen-qiang, LIU Kai-hui, DING Xiao-wei, GU Dong-sheng, LI Hua |
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| Abstract: | In this study , the tissue grinder method was used to isolate the endophytic fungus from Taxus chinensis stems and forty-five endophytic fungus were obtained .The strain producing taxol （ Tax-4） was identi-fied as Massaria based on morphology and molecule .Endophytic fungi were obtained from Taxus chinensis stems, and the strain Tax-4 was identified as Massaria.It is indicated that the endophytic fungus Massaria producing taxol was isolated from Taxus chinensis, which further enriched the resources of taxol-producing fun-gi . |
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| Keywords: | Taxus chinensis taxol endophytic fungus isolation and identification |
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