拟南芥一氧化氮相关突变体在干旱胁迫下抗旱相关基因表达的研究

据报道,拟南芥一氧化氮（nitric oxide,NO）合成缺陷突变体noa1较野生型Col-0抗旱,而个别抗旱相关基因表达的增强是其抗旱机理之一.为了证实该结果及研究NO信号分子在抗旱中的作用,系统地对8种共12个不同类型的抗旱相关基因在干旱处理不同时间的拟南芥野生型和noa1及亚硝基谷胱甘肽还原酶功能缺失突变体gsnor1-3间的表达情况进行了反转录PCR分析.研究结果表明,抗旱相关基因的表达在拟南芥不同基因型间无明显差异,noa1的耐旱性与抗旱相关基因的表达无显著相关性.

The expression analysis of drought tolerance-related genes in NO-related mutants under drought treatment conditions

About nitric oxide（ NO）,a small gaseous molecule that regulated many biological processes in plants,was reported that Arabidopsis noa1 mutant,defective in NO production,was more resistant to water deficit than wilde-type Col-0 and the higher expression levels of a few drought-related genes were correlated with the enhanced drought tolerance of noa1. To confirm the results and further investigate the role of NO in drought tolerance,the plants of Col-0,noa1 as well as gsnor1-3,a GSNOR1 loss-of-function mutant,defective in de-nitrosylation were subjected to drought treatment for various length of time and RT-PCR analysis was performed for total of 12 drought-related genes that belonged to 8 different gene families. The RT-PCR analysis indicated that no significant differences were observed for the expression of genes related to drought resistance /tolerance among Col-0,noa1 and gsnor1-3 and the drought tolerance displayed by noa1 might not result from higher expression of genes involved in drought tolerance.