~1I-抗膀胱癌单克隆抗体在荷瘤裸鼠的体内分布与放射免疫显像

用氯胺T法际记鼠抗人膀胱癌单克隆抗体(抗BIU-87McAb),标记率及标记抗体免疫活性满意。抗体注射于荷瘤裸鼠,靶与非靶放射性比值随肘间延长而增高,尾静脉注射后72h:肿瘤/血液=0.99、肿瘤/肌肉=8.43、肿瘤/膀胱壁=4.04;注射后120h:肿瘤/血液=1.38、肿瘤/肌肉=12.31、肿瘤/膀胱壁=5.24。于注射后72h行Y照相,肿瘤影像明显可见,5—7天影像更为清晰。与非特异性对照放免显像对比,表明抗BIU-87McAb具有高度特异牲,有可能进一步应用于人体膀胱癌及其转移灶的导向诊断与治疗。

DISTRIBUTION AND RADIOIMMUNOIMAGING OF ~(131)I-ANTI-HUMAN BLADDER CARCINOMA McAb IN NUDE MICE WITH TUMOR XENOGRAFTS

Anti human bladder carcinoma monoclonal antibody (Anti BIU-87 McAb) was radioiodinated using Ch-T method with satisfied lebelling efficiency and immune activity. After injection of the antibody into nude mice with tumor xenografts, the radioactivity ratio of target to nontarget tissues increased with time. 72 hours post intravenous injection, tumor/blood ratio was 0.99, tumor/muscle 8.43, tumor/bladder 4.04. 120 hours post injection, tumor/blood ratio was 1.38, tumor/muscle 12.31, tumor/bladder 5.24. Tumor imaging with γ camera was clear 3 days post injection, and it was mors clear during 5-7 days post injection. Contrasted with non-specific imaging indicated that anti BIU-87 McAb has high specificity. The results suggest that anti BIU-87 McAb be useful for guiding diagnosis and therapy of bladder carcer and its matastatic focus.