东方田鼠抗日本血吸虫病机制研究

本文报道了在东方田鼠抗日本血吸虫机制研究方面的研究成果。经多次重复感染试验 ,证实来源于血吸虫疫区 (洞庭湖 )和非疫区 (宁夏青铜峡 )的东方田鼠均具有抗日本血吸虫病能力 ,这种能力是可遗传的 ,与获得性免疫关系不大 ;体内外的检测与杀伤试验显示东方田鼠体内存在的抗日本血吸虫童虫和成虫抗原的天然抗体IgG3和补体可能在其抗血吸虫病方面具有很重要的作用 ;东方田鼠和腹腔巨噬细胞 (Mф)、嗜酸细胞 (Eos)对血吸虫童虫具有天然的黏附能力 ;用东方田鼠血清筛选日本血吸虫成虫cDNA文库 ,获得了 5个阳性克隆 ,其中 4个克隆为新基因 ,…

Study on the Mechanism of Microtus fortis Against Schistosoma Japonicum

This paper was about the study on the mechanism of Microtus fortis against Schistosoma japonicum. Firstly, we confirmed that Microtus fortis came from epidemic region (Dongting Lake beaches) and non epidemic region (Qingtong Gorge in Ningxia province) were both resistant to Schistosoma japonicum infection after re infection tests for several times. It seemed that their resistant ability was inheritable rather than acquired. Secondly, it was demonstrated by in vivo check up and in vitro killing assay that there were some native antibodies of IgG3 subclass specifically to the schistosomula and adult worm of Schistosoma japonicum in Microtus fortis, which probably played an important role in resisting Schistosoma japonicum associated with complement. It was shown that macrophages and eosinophils in abdominal cavity of Microtus fortis had native ability of adhering to the schistosomula of Schistosoma japonicum. Then, the adult worm cDNA library of Schistosoma japonicum was screened with sera from Microtus fortis . Five positive clones were obtained, four of which were identified as new genes. Full length cDNA of the two new genes were isolated by RACE. DNA vaccine was constructed with one named EST mfs 3. After the Kunming mice immunized with this vaccine, the worm reduction rate and the egg reduction rate were 28.4% and 21.73% compared with that in control group respectively. This kind of DNA based EST mfs 3 vaccine was highly expressed in E.coli and induecd strong immune response in challenged group. Finally, two groups of cDNA probes prepared from liver and lung of Microtus fortis with or without Schistosoma japonicum infection were hybridized to the cDNA chip prepared from rat respectively. 156 and 332 genes revealed differential expression in infectious group compared with normal group. In conclusion, there would be many factors contribute to the mechanism of Microtus fortis against Schistosoma japonicum. We should stress the essentials and make further research on how to take advantage of them to defend us from Schistosoma japonicum infection.