利用灌注技术观察昆明小鼠Willis环的形态结构

目的建立完整的昆明小鼠动脉环(Willis环)形态结构。方法选用昆明小鼠利用灌注技术经左心室往脑血管内灌注填充塑化剂约2 mL,去骨取脑,观察Willis环结构。结果该方法使复杂的小鼠脑血管及Willis环立体清晰的显露出来。结论我们采用灌注技术显示小鼠脑血管动脉环的形态结构,对小鼠脑血管的模型制作具有一定的指导意义。

Morphological Observation of Willis Ring Structure of the Kunming Mouse by Using a Perfusion Technique

To establish a fine morphological structure of Kunming mice arterial ring （Willis ring）.Select Kunming mice by perfusion technology.Briefly infusing plasticizer about 2 ml from the left ventricle to cerebral vessel was performed,then skull bones were removed,and the structure of Willis ring was observed.This method made a fine cerebral vessel very clear and Willis ring clearly was revealed in the three-dimensional.We successfully adopted the perfusion technology to show the morphological structure of mice cerebral vessel which would play an important role for manufacturing the model of mice cerebral vessel.