| 利用双向电泳-质谱技术鉴定牦牛和犏牛睾丸中的差异表达蛋白质 |
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| 引用本文： | 付伟,李彩霞,刘文静,马晓琴,任亮,黄林,金素钰,郑玉才.利用双向电泳-质谱技术鉴定牦牛和犏牛睾丸中的差异表达蛋白质[J].西南民族学院学报(自然科学版),2014(1):11-15. |
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| 作者姓名： | 付伟 李彩霞 刘文静 马晓琴 任亮 黄林 金素钰 郑玉才 |
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| 作者单位： | [1]西南民族大学生命科学与技术学院,成都610041 [2]西南科技大学生命科学与工程学院,绵阳621010 |
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| 基金项目： | 国家自然科学基金资助课题（No.31240053）;中央高校基本科研业务费资助课题（No.12NZYTH06）;西南民族大学研究生创新型科研项目资助（No.CX2013SZ52）. |
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| 摘 要： | 比较了牦牛和犏牛睾丸组织中蛋白质的表达差异;应用双向电泳-质谱技术,对牦牛和犏牛的睾丸组织中差异蛋白质进行分离和鉴定;对获得的22个差异表达点进行质谱鉴定和数据库搜索,获得了19个蛋白质,其中有3种分子伴侣可能与犏牛雄性不育有关;研究结果表明,牦牛和犏牛睾丸组织中存在差异表达蛋白,并可利用双向凝胶电泳进行分离,为进一步分析犏牛雄性不育的原因提供了线索.
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| 关 键 词： | 牦牛 犏牛 睾丸 双向电泳 质谱 |
Identification of the differentially expressed testis proteins between yaks and cattle-yaks by two-dimensional electrophoresis combined with mass spectrometry |
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| FU Wei,LI Cai-xia,LIU Wen-jing,MA Xiao-qin,REN Liang,HUANG Lin,JIN Su-yu,LIN Ya-qiu,ZHENG Yu-cai.Identification of the differentially expressed testis proteins between yaks and cattle-yaks by two-dimensional electrophoresis combined with mass spectrometry[J].Journal of Southwest Nationalities College(Natural Science Edition),2014(1):11-15. |
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| Authors: | FU Wei LI Cai-xia LIU Wen-jing MA Xiao-qin REN Liang HUANG Lin JIN Su-yu LIN Ya-qiu ZHENG Yu-cai |
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| Institution: | 1. School of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, ER.C.; 2. School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, P.R.C.) |
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| Abstract: | The objective of this study is to analyze the diifferently expressed testis proteins between yaks and cattle-yaks. Two-dimensional electrophoresis is used to isolate the proteins, and the differential protein spots are identified by mass spectrometry. Twenty-two differential proteins are succe,~sfully identified by mass spectrometry, and the corresponding nineteen proteins, including three chaperons which may relate to the male sterility of cattle-yaks, are obtained successfully. The present results show that two-dimensional electrophor~,~sis combined with mass spectrometry can be used to analyze the expression dift~rence of proteins between yak and cattle-yak testes, helping to elucidate the molecular mechanism of infertility in male cattle-yaks. |
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| Keywords: | yak cattle-yak testis two-dimensional electrophoresis mass spectrometry |
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