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| 禽流感病毒H5亚型特异性抗原捕捉ELISA检测方法的研究 | |
| 引用本文： | 张文东,宋建领,王金萍,李作生,冯子良,胡媛媛,郭松辉,张应国,范泉水,宋学林,邱薇,张富强.禽流感病毒H5亚型特异性抗原捕捉ELISA检测方法的研究[J].云南大学学报(自然科学版),2007,29(6):633-637. |
| 作者姓名： | 张文东 宋建领 王金萍 李作生 冯子良 胡媛媛 郭松辉 张应国 范泉水 宋学林 邱薇 张富强 |
| 作者单位： | 1. 云南省动物疫病预防控制中心,云南,昆明,650051 2. 云南省热带亚热带动物病毒病重点实验室,云南,昆明,650224 3. 成都军区疾病预防控制中心,云南,昆明,650032 |
| 基金项目： | 云南省科技攻关计划，昆明市科技重点项目，云南省跨世纪人才培养基金 |
| 摘 要： | 在获得禽流感病毒多克隆抗体及H5亚型特异性单克隆抗体的基础上,研究建立H5亚型特异性抗原捕捉ELISA检测方法,用于检测H5亚型禽流感病毒.优化反应条件,确定包被抗体、检测抗体及酶结合物的最佳工作浓度,进行敏感性、特异性、重复性及稳定性分析,并与RT-PCR方法比较.同时使用该方法对野外样品进行检测.结果表明:该方法敏感、特异,具有良好的重复性和稳定性,可用于检测临床样品、鸡胚培养物及细胞培养物中的H5亚型禽流感病毒. |
| 关 键 词： | 禽流感病毒 H5亚型特异性 抗原捕捉ELISA |
| 文章编号： | 0258-7971（2007）06-0633-05 |
| 修稿时间： | 2007-03-28 |
Development of subtype-specific antigen capture ELISA for detection of H5 subtype avian influenza virus |
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| ZHANG Wen-dong,SONG Jian-ling,WANG Jin-ping,LI Zuo-sheng,FENG Zi-liang,HU Yuan-yuan,GUO Song-hui,ZHANG Ying-guo,FAN Quan-shui,SONG Xue-lin,QIU Wei,ZHANG Fu-qiang.Development of subtype-specific antigen capture ELISA for detection of H5 subtype avian influenza virus[J].Journal of Yunnan University(Natural Sciences),2007,29(6):633-637. | |
| Authors: | ZHANG Wen-dong SONG Jian-ling WANG Jin-ping LI Zuo-sheng FENG Zi-liang HU Yuan-yuan GUO Song-hui ZHANG Ying-guo FAN Quan-shui SONG Xue-lin QIU Wei ZHANG Fu-qiang |
| Institution: | 1. Yunnan General Veterinary Station, Kunming 650051, China; 2. Yunnan Tropical and Subtropical Animal Virus Diseases Laboratory, Kunming 650224, China; 3. Centre for Disease Prevention and Control, Chengdu Military Region, Kunming 650032, China |
| Abstract: | Based on obtaining avian influenza polyclonal antibody and H5 subtype-specific monoclonal antibody,a H5 subtype specific antigen capture ELISA was developed for detection of H5 subtype avian influenza virus.The reactive condition and the working titers of coating antibody,detective antibody and enzyme-labeled conjugate were optimized,and the assay sensitivity,specificity,repeatability,stability were analyzed and compared with virus isolation and identification.The results indicated that antigen capture ELISA was sensitive,specific,with high repeatability and stability for detection of H5 subtype avian influenza virus in clinical samples,embryo and cell culture tissues. |
| Keywords: | avian influenza virus H5 subtype-specific antigen capture ELISA |
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