| 树舌、茯苓多糖的提取分离及组成 |
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| 引用本文： | 连宾,郁建平.树舌、茯苓多糖的提取分离及组成[J].重庆大学学报(自然科学版),2004,27(1):120-122. |
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| 作者姓名： | 连宾 郁建平 |
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| 作者单位： | [1]贵州工业大学化学与生物工程学院，贵阳550003 [2]贵州大学生化营养研究所，贵州贵阳550025 |
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| 基金项目： | 教育部西部地区高校高级访问学者计划项目 |
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| 摘 要： | 对树舌(Ganoderma apparatus)、茯苓(poria cocos)多糖的提取、纯化和单糖的组成成分进行了研究.认为树舌子实体和茯苓菌核经热水提取、脱色和乙醇沉淀分别得到相应多糖(树舌多糖Ga,茯苓多糖Pc)粗品,多糖粗品经脱蛋白,乙醇分级沉淀、Sephadex G-15葡聚糖凝胶柱层析纯化得Ga-1、Ga-2、Ga-3和Pc -1、Pc -2、Pc -3 3个级分,并应用薄板层析技术,确定树舌多糖3个级分均由葡萄糖、树胶醛糖、甘露糖、果糖及一种未知单糖组成,茯苓多糖3个级分均由葡萄糖、甘露糖、核糖及一种未知单糖组成.
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| 关 键 词： | 树舌 茯苓 多糖 提取 组成 |
| 文章编号： | 1000-582X(2004)01-0120-03 |
| 修稿时间： | 2003年10月11日 |
The Extraction,Isolation and the Composition of Polysaccharides From Ganoderma Apparatus and Poria Cocos |
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| LIAN Bin.The Extraction,Isolation and the Composition of Polysaccharides From Ganoderma Apparatus and Poria Cocos[J].Journal of Chongqing University(Natural Science Edition),2004,27(1):120-122. |
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| Authors: | LIAN Bin~ |
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| Abstract: | This paper reports that the crude polysaccharides were extracted with hot water and precipitated with alcohol from the fruitbody of mushroom Ganoderma apparatus and Poria cocos. The free proteins were removed from crude polysaccharides by sevag method, and then the polysaccharides were isolated and purified by Sephadex G-15 gel column chromatography. Each three pure polysaccharides (Ga-1,Ga-2,Ga-3 and Pc-1,Pc-2,Pc-3) were obtained by precipitating with different concentration alcohol. By using the method of TLC, we proved that three fractions of Ganoderma apparatus polysaccharides are composed of glucose (Glu), arabinose (Ara), mannose (Man), fructose(Fru),et al. and the three fractions of Poria cocos polysaccharides are composed of glucose (Glu), mannose (Man) and ribose (Rib),et al. |
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| Keywords: | ganoderma apparatus poria cocos polysaccharides extraction composition P |
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