| 一种家蝇幼虫热稳定抗菌肽的分离纯化 |
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| 引用本文： | 盛长忠,安春菊,耿华,张一鸣,李德森,杜荣骞,纪明耀,李洪俊,周嘉伟.一种家蝇幼虫热稳定抗菌肽的分离纯化[J].南开大学学报,2002,35(4):6-10. |
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| 作者姓名： | 盛长忠 安春菊 耿华 张一鸣 李德森 杜荣骞 纪明耀 李洪俊 周嘉伟 |
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| 作者单位： | [1]南开大学生命科学学院，天津300071 [2]大港油田集团有限责任公司300280 |
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| 摘 要： | 本文对家蝇三龄幼虫进行了超声处理，以诱导其产生抗菌物质。通过高温处理、酸性处理后，抗菌物质粗提中大量的热不稳定蛋白和酸性蛋白发生沉淀而被去除。将经过上述处理的粗提液经过一步Sephadex G-75凝胶过滤析、两步CM－Sepharose阳离子交换层析后，得到一个单一的具有抗阳性球菌的峰。SDS－聚丙烯酰胺凝胶电泳结果显示为一条带，相对分子质量大约为7000，将其命名为H1．H1为一种热稳定的碱性抗菌多肽。
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| 关 键 词： | 家蝇 幼虫 热稳定 抗菌肽 分离 纯化 凝胶过滤层析 离子交换层析 SDS－聚丙烯酰胺凝胶电泳 抗菌活性 |
| 文章编号： | 0465-7942(2002)04-0006-05 |
SEPARATION AND PURIFICATION OF A HEAT-STABLE ANTIBACTERIAL PEPTIDE OF THE LARVAE OF HOUSEFLY |
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| SHENG Changzhong ,AN Chunju ,GENG Hua ,ZHANG Yiming ,LI Desen ,DU Rongqian ,JI Mingyao ,LI Hongjun ,ZHOU Jiawei.SEPARATION AND PURIFICATION OF A HEAT-STABLE ANTIBACTERIAL PEPTIDE OF THE LARVAE OF HOUSEFLY[J].Acta Scientiarum Naturalium University Nankaiensis,2002,35(4):6-10. |
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| Authors: | SHENG Changzhong AN Chunju GENG Hua ZHANG Yiming LI Desen DU Rongqian JI Mingyao LI Hongjun ZHOU Jiawei |
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| Institution: | SHENG Changzhong 1,AN Chunju 1,GENG Hua 1,ZHANG Yiming 1,LI Desen 1,DU Rongqian 1,JI Mingyao 2,LI Hongjun 2,ZHOU Jiawei |
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| Abstract: | In this paper, antibacterial substances of the larvae of housefly were induced by ultrasonic treatment. Heating and acid treated the antibacterial crude extracts and many heat unstable proteins and acid proteins were excluded. Then, the antibacterial crude extracts were purified using Sephadex G 75 gel chromatography and two steps CM Sepharose ion exchange chromatography. The samples were analyzed by SDS PAGE. The results indicated that an antibacterial substance was purified, which was small molecular (about 7000Da), heat stable, alkaline peptide. The peptide was named H1.$$$$ |
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| Keywords: | housefly (Musca domestica) antibacterial peptide gel chromatography ion exchange chromatography SDS-PAGE |
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